biostudies-literatureBicer FNIDDK NIH HHS490.e1-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC4114992biostudies-literatureUnknown84(2)<h4>Objective</h4>To develop and test the efficacy of an implantable bladder electrode device that can be used with the Neurometer electrodiagnostic stimulator to assess fiber-specific afferent bladder sensation in the mouse.<h4>Methods</h4>We constructed a ball-tipped platinum electrode and surgically implanted it into the mouse bladder. The Neurometer was connected to the electrode to apply selective nerve fiber stimuli (250 Hz for Aδ fibers and 5 Hz for C fibers) of increasing intensities to the bladder mucosa in the mouse to determine bladder sensory threshold (BST) values. Using 58 female C57BL/6J mice, we measured the temporal and interobserver consistency of BST measurements, the effects of intravesical administration of lidocaine and resiniferatoxin on the BST, and the effects of oUrologyAssessment of bladder sensation in mice with a novel device.PMC4114992R01 DK083733Horowitz ADaneshgari FBicer FKim JYLiu GfalseAssessment of bladder sensation in mice with a novel device.<h4>Objective</h4>To develop and test the efficacy of an implantable bladder electrode device that can be used with the Neurometer electrodiagnostic stimulator to assess fiber-specific afferent bladder sensation in the mouse.<h4>Methods</h4>We constructed a ball-tipped platinum electrode and surgically implanted it into the mouse bladder. The Neurometer was connected to the electrode to apply selective nerve fiber stimuli (250 Hz for Aδ fibers and 5 Hz for C fibers) of increasing intensities to the bladder mucosa in the mouse to determine bladder sensory threshold (BST) values. Using 58 female C57BL/6J mice, we measured the temporal and interobserver consistency of BST measurements, the effects of intravesical administration of lidocaine and resiniferatoxin on the BST, and the effects of o2014-01-01T00:00:00Z2014 Aug2025-04-18T23:43:59.247Z2019-03-27T01:33:01ZS-EPMC41149922495848510.1016/j.urology.2014.04.024