{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dang LT"],"funding":["NSERC-CIHR Collaborative Health Research","NHLBI NIH HHS","NSERC Discovery Accelerator Supplement","Canadian Institutes of Health Research (CIHR) Operating","National Institutes of Health","Heart and Stoke Foundation GIA","NSERC Discovery","Connaught Innovation Award","CIHR","Ontario Research Fund–Global Leadership Round 2"],"pagination":["7786-99"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4117357"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(27)"],"pubmed_abstract":["Adhesion molecule signaling is critical to human pluripotent stem cell (hPSC) survival, self-renewal, and differentiation. Thus, hPSCs are grown as clumps of cells on feeder cell layers or poorly defined extracellular matrices such as Matrigel. We sought to define a small molecule that would initiate adhesion-based signaling to serve as a basis for a defined substrate for hPSC culture. Soluble angiopoeitin-1 (Ang-1)-derived peptide QHREDGS added to defined serum-free media increased hPSC colony cell number and size during long- and short-term culture when grown on feeder cell layers or Matrigel, i.e. on standard substrates, without affecting hPSC morphology, growth rate or the ability to differentiate into multiple lineages both in vitro and in vivo. Importantly, QHREDGS treatment decrease"],"journal":["Biomaterials"],"pubmed_title":["Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS."],"pmcid":["PMC4117357"],"funding_grant_id":["CHRPJ 385981-10","T 6946","385981-10","2R01 HL076485","MOP-126027","RGPAS 396125-10","RGPIN 326982-10","R01 HL076485"],"pubmed_authors":["Chang WY","Dang LT","Radisic M","Stanford WL","Feric NT","Laschinger C","Zhang B","Wood GA"],"additional_accession":[]},"is_claimable":false,"name":"Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS.","description":"Adhesion molecule signaling is critical to human pluripotent stem cell (hPSC) survival, self-renewal, and differentiation. Thus, hPSCs are grown as clumps of cells on feeder cell layers or poorly defined extracellular matrices such as Matrigel. We sought to define a small molecule that would initiate adhesion-based signaling to serve as a basis for a defined substrate for hPSC culture. Soluble angiopoeitin-1 (Ang-1)-derived peptide QHREDGS added to defined serum-free media increased hPSC colony cell number and size during long- and short-term culture when grown on feeder cell layers or Matrigel, i.e. on standard substrates, without affecting hPSC morphology, growth rate or the ability to differentiate into multiple lineages both in vitro and in vivo. Importantly, QHREDGS treatment decrease","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Sep","modification":"2025-04-03T23:18:12.042Z","creation":"2019-03-27T01:33:07Z"},"accession":"S-EPMC4117357","cross_references":{"pubmed":["24930852"],"doi":["10.1016/j.biomaterials.2014.05.018"]}}