{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liefhebber JM"],"funding":["Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["21276-88"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4118089"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["289(31)"],"pubmed_abstract":["In hepatitis C virus infection, replication of the viral genome and virion assembly are linked to cellular metabolic processes. In particular, lipid droplets, which store principally triacylglycerides (TAGs) and cholesterol esters (CEs), have been implicated in production of infectious virus. Here, we examine the effect on productive infection of triacsin C and YIC-C8-434, which inhibit synthesis of TAGs and CEs by targeting long-chain acyl-CoA synthetase and acyl-CoA:cholesterol acyltransferase, respectively. Our results present high resolution data on the acylglycerol and cholesterol ester species that were affected by the compounds. Moreover, triacsin C, which blocks both triglyceride and cholesterol ester synthesis, cleared most of the lipid droplets in cells. By contrast, YIC-C8-434, "],"journal":["The Journal of biological chemistry"],"pubmed_title":["Modulation of triglyceride and cholesterol ester synthesis impairs assembly of infectious hepatitis C virus."],"pmcid":["PMC4118089"],"funding_grant_id":["MC_UU_12014/1","BBS/E/B/000C0415","BBS/E/B/0000S227"],"pubmed_authors":["McLauchlan J","Wakelam MJ","Hague CV","Liefhebber JM","Zhang Q"],"additional_accession":[]},"is_claimable":false,"name":"Modulation of triglyceride and cholesterol ester synthesis impairs assembly of infectious hepatitis C virus.","description":"In hepatitis C virus infection, replication of the viral genome and virion assembly are linked to cellular metabolic processes. In particular, lipid droplets, which store principally triacylglycerides (TAGs) and cholesterol esters (CEs), have been implicated in production of infectious virus. Here, we examine the effect on productive infection of triacsin C and YIC-C8-434, which inhibit synthesis of TAGs and CEs by targeting long-chain acyl-CoA synthetase and acyl-CoA:cholesterol acyltransferase, respectively. Our results present high resolution data on the acylglycerol and cholesterol ester species that were affected by the compounds. Moreover, triacsin C, which blocks both triglyceride and cholesterol ester synthesis, cleared most of the lipid droplets in cells. By contrast, YIC-C8-434, ","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Aug","modification":"2025-04-04T10:07:59.998Z","creation":"2019-03-27T01:33:12Z"},"accession":"S-EPMC4118089","cross_references":{"pubmed":["24917668"],"doi":["10.1074/jbc.M114.582999"]}}