{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(7)"],"submitter":["Aoi Y"],"pubmed_abstract":["Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays meiotic defects, and is sensitive to high and low temperature even in the absence of ATP-analogue inhibitors. This has limited the use of the strain for use in these settings. Here, we used in vivo selection for intragenic suppressor mutations of cdc2-as that restore full function in the absence of ATP-analogues. The cdc2-asM17 underwent meiosis and produced viable spores to a similar degree to the wild-type strain. The suppressor mutation also rescued the sensitivity of the cdc2-as strain to high and low temperature"],"journal":["Open biology"],"pagination":["140063"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4118601"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis."],"pmcid":["PMC4118601"],"pubmed_authors":["Aoi Y","Yamamoto M","Sato M","Kawashima SA","Simanis V"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.","description":"Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays meiotic defects, and is sensitive to high and low temperature even in the absence of ATP-analogue inhibitors. This has limited the use of the strain for use in these settings. Here, we used in vivo selection for intragenic suppressor mutations of cdc2-as that restore full function in the absence of ATP-analogues. The cdc2-asM17 underwent meiosis and produced viable spores to a similar degree to the wild-type strain. The suppressor mutation also rescued the sensitivity of the cdc2-as strain to high and low temperature","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2025-04-04T10:08:33.009Z","creation":"2019-03-27T01:33:13Z"},"accession":"S-EPMC4118601","cross_references":{"pubmed":["24990387"],"doi":["10.1098/rsob.140063"]}}