{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Smith EM"],"funding":["NIGMS NIH HHS","PHS HHS"],"pagination":["66-75"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4119281"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["107(1)"],"pubmed_abstract":["Fluorescently labeled proteins that are found both in the cytoplasm and at the plasma membrane, such as peripheral membrane proteins, create stratified fluorescent layers that present a challenging environment for brightness studies with fluorescence fluctuation spectroscopy. The geometry of each layer along with fluorescence and brightness contributions from adjacent layers generates a convoluted raw brightness that conceals the underlying brightness of each individual layer. Because the brightness at a layer establishes the oligomeric state of the fluorescently labeled protein at said layer, we developed a method that connects the experimental raw brightness with the physical brightness at each layered compartment. The technique determines the oligomerization in each compartment from an "],"journal":["Biophysical journal"],"pubmed_title":["Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis."],"pmcid":["PMC4119281"],"funding_grant_id":["5T32A1083196","R01 GM064589","R01GM064589"],"pubmed_authors":["Mueller JD","Chen Y","Macdonald PJ","Smith EM"],"additional_accession":[]},"is_claimable":false,"name":"Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis.","description":"Fluorescently labeled proteins that are found both in the cytoplasm and at the plasma membrane, such as peripheral membrane proteins, create stratified fluorescent layers that present a challenging environment for brightness studies with fluorescence fluctuation spectroscopy. The geometry of each layer along with fluorescence and brightness contributions from adjacent layers generates a convoluted raw brightness that conceals the underlying brightness of each individual layer. Because the brightness at a layer establishes the oligomeric state of the fluorescently labeled protein at said layer, we developed a method that connects the experimental raw brightness with the physical brightness at each layered compartment. The technique determines the oligomerization in each compartment from an ","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2025-04-19T04:17:45.341Z","creation":"2019-03-27T01:33:15Z"},"accession":"S-EPMC4119281","cross_references":{"pubmed":["24988342"],"doi":["10.1016/j.bpj.2014.04.055"]}}