biostudies-literatureDavies DHNIAID NIH HHS1812-27https://www.ebi.ac.uk/biostudies/studies/S-EPMC4119580biostudies-literatureUnknown193(4)Vaccinia virus (VACV) is a useful model system for understanding the immune response to a complex pathogen. Proteome-wide Ab profiling studies reveal the humoral response to be strongly biased toward virion-associated Ags, and several membrane proteins induce Ab-mediated protection against VACV challenge in mice. Some studies have indicated that the CD4 response is also skewed toward proteins with virion association, whereas the CD8 response is more biased toward proteins with early expression. In this study, we have leveraged a VACV strain Western Reserve (VACV-WR) plasmid expression library, produced previously for proteome microarrays for Ab profiling, to make a solubilized full VACV-WR proteome for T cell Ag profiling. Splenocytes from VACV-WR-infected mice were assayed without prior eJournal of immunology (Baltimore, Md. : 1950)T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and nonvirion association.PMC4119580R43 AI063728R44 AI058365R43AI063728U54 AI065359U54AI065359Tucker JAJain AChun SFelgner PLLiang XDavies DHHermanson GNakajima RPablo JfalseT cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and nonvirion association.Vaccinia virus (VACV) is a useful model system for understanding the immune response to a complex pathogen. Proteome-wide Ab profiling studies reveal the humoral response to be strongly biased toward virion-associated Ags, and several membrane proteins induce Ab-mediated protection against VACV challenge in mice. Some studies have indicated that the CD4 response is also skewed toward proteins with virion association, whereas the CD8 response is more biased toward proteins with early expression. In this study, we have leveraged a VACV strain Western Reserve (VACV-WR) plasmid expression library, produced previously for proteome microarrays for Ab profiling, to make a solubilized full VACV-WR proteome for T cell Ag profiling. Splenocytes from VACV-WR-infected mice were assayed without prior e2014-01-01T00:00:00Z2014 Aug2026-04-30T01:13:40.683Z2019-03-27T01:33:16ZS-EPMC41195802502439210.4049/jimmunol.1400663