{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dixit S"],"funding":["NIGMS NIH HHS"],"pagination":["1311-21"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4119847"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["PLA-PEG [poly(lactic acid)-poly (ethylene glycol)], a biodegradable copolymer, is underexploited for vaccine delivery although it exhibits enhanced biocompatibility and slow release immune-potentiating properties. We document here successful encapsulation of M278, a Chlamydia trachomatis MOMP (major outer-membrane protein) peptide, within PLA-PEG nanoparticles by size (~73-100nm), zeta potential (-16 mV), smooth morphology, encapsulation efficiency (~60%), slow release pattern, and non-toxicity to macrophages. Immunization of mice with encapsulated M278 elicited higher M278-specific T-cell cytokines [Th1 (IFN-γ, IL-2), Th17 (IL-17)] and antibodies [Th1 (IgG2a), Th2 (IgG1, IgG2b)] compared to bare M278. Encapsulated-M278 mouse serum inhibited Chlamydia infectivity of macrophages, with a con"],"journal":["Nanomedicine : nanotechnology, biology, and medicine"],"pubmed_title":["Poly(lactic acid)-poly(ethylene glycol) nanoparticles provide sustained delivery of a Chlamydia trachomatis recombinant MOMP peptide and potentiate systemic adaptive immune responses in mice."],"pmcid":["PMC4119847"],"funding_grant_id":["1R25GM106995-01","R25 GM106995"],"pubmed_authors":["Dixit S","Yilma AN","Dennis VA","Singh SR","Agee RD","Taha M"],"additional_accession":[]},"is_claimable":false,"name":"Poly(lactic acid)-poly(ethylene glycol) nanoparticles provide sustained delivery of a Chlamydia trachomatis recombinant MOMP peptide and potentiate systemic adaptive immune responses in mice.","description":"PLA-PEG [poly(lactic acid)-poly (ethylene glycol)], a biodegradable copolymer, is underexploited for vaccine delivery although it exhibits enhanced biocompatibility and slow release immune-potentiating properties. We document here successful encapsulation of M278, a Chlamydia trachomatis MOMP (major outer-membrane protein) peptide, within PLA-PEG nanoparticles by size (~73-100nm), zeta potential (-16 mV), smooth morphology, encapsulation efficiency (~60%), slow release pattern, and non-toxicity to macrophages. Immunization of mice with encapsulated M278 elicited higher M278-specific T-cell cytokines [Th1 (IFN-γ, IL-2), Th17 (IL-17)] and antibodies [Th1 (IgG2a), Th2 (IgG1, IgG2b)] compared to bare M278. Encapsulated-M278 mouse serum inhibited Chlamydia infectivity of macrophages, with a con","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Aug","modification":"2025-04-19T04:17:31.228Z","creation":"2019-03-27T01:33:17Z"},"accession":"S-EPMC4119847","cross_references":{"pubmed":["24602605"],"doi":["10.1016/j.nano.2014.02.009"]}}