{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim N"],"funding":["Ministry of Education","National Cancer Institute","Ministry of Health and Welfare","NCI NIH HHS","National Research Foundation of Korea","National Institutes of Health","Korea Research Institute of Bioscience and Biotechnology"],"pagination":["195-203"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4125537"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["134(1)"],"pubmed_abstract":["<h4>Background</h4>Perforin-1 (Prf1) is the predominant cytolytic protein secreted by natural killer (NK) cells. For a rapid immune response, resting NK cells contain high Prf1 mRNA concentrations while exhibiting minimal cytotoxicity caused by a blockage of Prf1 protein synthesis, implying that an unknown posttranscriptional regulatory mechanism exists.<h4>Objective</h4>We sought to determine whether microRNA-150 (miR-150) posttranscriptionally regulates Prf1 translation in both mouse and human NK cells at rest and at various time points after activation.<h4>Methods</h4>Mouse NK cells with a targeted deletion of miR-150 (miR-150(-/-) NK cells), primary human NK cells, and NK92 MI cells were used to investigate the role of miR-150 in NK cells. NK cell cytotoxicity assays and Western blotting proved that activated miR-150(-/-) NK cells expressed upregulated Prf1, augmenting NK cell cytotoxicity. When immunodeficient mice were injected with miR-150(-/-) NK cells, there was a significant reduction in tumor growth and metastasis of B16F10 melanoma.<h4>Results</h4>We report that miR-150 binds to 3' untranslated regions of mouse and human Prf1, posttranscriptionally downregulating its expression. Mouse wild-type NK cells displayed downregulated miR-150 expression in response to IL-15, which led to corresponding repression and induction of Prf1 during rest and after IL-15 activation, respectively.<h4>Conclusion</h4>Our results indicate that miR-150 is a common posttranscriptional regulator for Prf1 in mouse and human NK cells that represses NK cell lytic activity. Thus the therapeutic control of miR-150 in NK cells could enhance NK cell-based immunotherapy against cancer, providing a better clinical outcome."],"journal":["The Journal of allergy and clinical immunology"],"pubmed_title":["MicroRNA-150 regulates the cytotoxicity of natural killers by targeting perforin-1."],"pmcid":["PMC4125537"],"funding_grant_id":["A121934","RBM0261312","R37 CA033084","CA33084","R01 CA033084"],"pubmed_authors":["Greenberg PD","Kim TD","Doh J","Yun S","Choi I","Kim M","Kim N"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-150 regulates the cytotoxicity of natural killers by targeting perforin-1.","description":"<h4>Background</h4>Perforin-1 (Prf1) is the predominant cytolytic protein secreted by natural killer (NK) cells. For a rapid immune response, resting NK cells contain high Prf1 mRNA concentrations while exhibiting minimal cytotoxicity caused by a blockage of Prf1 protein synthesis, implying that an unknown posttranscriptional regulatory mechanism exists.<h4>Objective</h4>We sought to determine whether microRNA-150 (miR-150) posttranscriptionally regulates Prf1 translation in both mouse and human NK cells at rest and at various time points after activation.<h4>Methods</h4>Mouse NK cells with a targeted deletion of miR-150 (miR-150(-/-) NK cells), primary human NK cells, and NK92 MI cells were used to investigate the role of miR-150 in NK cells. NK cell cytotoxicity assays and Western blotting proved that activated miR-150(-/-) NK cells expressed upregulated Prf1, augmenting NK cell cytotoxicity. When immunodeficient mice were injected with miR-150(-/-) NK cells, there was a significant reduction in tumor growth and metastasis of B16F10 melanoma.<h4>Results</h4>We report that miR-150 binds to 3' untranslated regions of mouse and human Prf1, posttranscriptionally downregulating its expression. Mouse wild-type NK cells displayed downregulated miR-150 expression in response to IL-15, which led to corresponding repression and induction of Prf1 during rest and after IL-15 activation, respectively.<h4>Conclusion</h4>Our results indicate that miR-150 is a common posttranscriptional regulator for Prf1 in mouse and human NK cells that represses NK cell lytic activity. Thus the therapeutic control of miR-150 in NK cells could enhance NK cell-based immunotherapy against cancer, providing a better clinical outcome.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2025-04-22T04:41:32.52Z","creation":"2019-03-27T01:33:39Z"},"accession":"S-EPMC4125537","cross_references":{"pubmed":["24698324"],"doi":["10.1016/j.jaci.2014.02.018"]}}