biostudies-literatureNakajima ECHoward Hughes Medical InstituteNCI NIH HHSe102452https://www.ebi.ac.uk/biostudies/studies/S-EPMC4134191biostudies-literatureUnknown9(8)<h4>Purpose</h4>Intratumoral metabolic heterogeneity may increase the likelihood of treatment failure due to the presence of a subset of resistant tumor cells. Using a head and neck squamous cell carcinoma (HNSCC) xenograft model and a real-time fluorescence imaging approach, we tested the hypothesis that tumors are metabolically heterogeneous, and that tumor hypoxia alters patterns of glucose uptake within the tumor.<h4>Experimental design</h4>Cal33 cells were grown as xenograft tumors (n = 16) in nude mice after identification of this cell line's metabolic response to hypoxia. Tumor uptake of fluorescent markers identifying hypoxia, glucose import, or vascularity was imaged simultaneously using fluorescent molecular tomography. The variability of intratumoral 2-deoxyglucose (IR800-2-DG) PloS oneQuantifying metabolic heterogeneity in head and neck tumors in real time: 2-DG uptake is highest in hypoxic tumor regions.PMC4134191R01CA077308P30 CA047904P50 CA097190U01 CA140230U01CA140230R01 CA077308P30CA047904P50CA097190Mountz JMFerris RLGrandis JROborski MVan Houten BNakajima ECLaymon CHou WWang LfalseQuantifying metabolic heterogeneity in head and neck tumors in real time: 2-DG uptake is highest in hypoxic tumor regions.<h4>Purpose</h4>Intratumoral metabolic heterogeneity may increase the likelihood of treatment failure due to the presence of a subset of resistant tumor cells. Using a head and neck squamous cell carcinoma (HNSCC) xenograft model and a real-time fluorescence imaging approach, we tested the hypothesis that tumors are metabolically heterogeneous, and that tumor hypoxia alters patterns of glucose uptake within the tumor.<h4>Experimental design</h4>Cal33 cells were grown as xenograft tumors (n = 16) in nude mice after identification of this cell line's metabolic response to hypoxia. Tumor uptake of fluorescent markers identifying hypoxia, glucose import, or vascularity was imaged simultaneously using fluorescent molecular tomography. The variability of intratumoral 2-deoxyglucose (IR800-2-DG) 2014-01-01T00:00:00Z20142026-04-07T22:43:59.144Z2025-05-29T22:29:25.823ZS-EPMC41341912512737810.1371/journal.pone.0102452