{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sauter KA"],"funding":["Cancer Research UK","Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["e105429"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4138162"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(8)"],"pubmed_abstract":["The MacBlue transgenic mouse uses the Csf1r promoter and first intron to drive expression of gal4-VP16, which in turn drives a cointegrated gal4-responsive UAS-ECFP cassette. The Csf1r promoter region used contains a deletion of a 150 bp conserved region covering trophoblast and osteoclast-specific transcription start sites. In this study, we examined expression of the transgene in embryos and adult mice. In embryos, ECFP was expressed in the large majority of macrophages derived from the yolk sac, and as the liver became a major site of monocytopoiesis. In adults, ECFP was detected at high levels in both Ly6C+ and Ly6C- monocytes and distinguished them from Ly6C+, F4/80+, CSF1R+ immature myeloid cells in peripheral blood. ECFP was also detected in the large majority of microglia and Lange"],"journal":["PloS one"],"pubmed_title":["The MacBlue binary transgene (csf1r-gal4VP16/UAS-ECFP) provides a novel marker for visualisation of subsets of monocytes, macrophages and dendritic cells and responsiveness to CSF1 administration."],"pmcid":["PMC4138162"],"funding_grant_id":["MR/L003384/1","BBS/E/D/20251969","G0901193","C157/A15703","BB/J014672/1","BBS/E/D/20221657","BB/J01666X/1","15703"],"pubmed_authors":["Donaldson DS","Davies CL","Frame MC","Mabbott NA","Scott C","Moffat L","Bain CC","Pridans C","McColl BW","Sauter KA","Serrels A","Hume DA","Rojo R","Mowat AM","Sehgal A","Stutchfield BM","Renault K"],"additional_accession":[]},"is_claimable":false,"name":"The MacBlue binary transgene (csf1r-gal4VP16/UAS-ECFP) provides a novel marker for visualisation of subsets of monocytes, macrophages and dendritic cells and responsiveness to CSF1 administration.","description":"The MacBlue transgenic mouse uses the Csf1r promoter and first intron to drive expression of gal4-VP16, which in turn drives a cointegrated gal4-responsive UAS-ECFP cassette. The Csf1r promoter region used contains a deletion of a 150 bp conserved region covering trophoblast and osteoclast-specific transcription start sites. In this study, we examined expression of the transgene in embryos and adult mice. In embryos, ECFP was expressed in the large majority of macrophages derived from the yolk sac, and as the liver became a major site of monocytopoiesis. In adults, ECFP was detected at high levels in both Ly6C+ and Ly6C- monocytes and distinguished them from Ly6C+, F4/80+, CSF1R+ immature myeloid cells in peripheral blood. ECFP was also detected in the large majority of microglia and Lange","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-22T17:57:57.434Z","creation":"2019-03-26T23:26:54Z"},"accession":"S-EPMC4138162","cross_references":{"pubmed":["25137049"],"doi":["10.1371/journal.pone.0105429"]}}