{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin YH"],"funding":["Intramural NIH HHS","National Institute on Alcohol Abuse and Alcoholism","National Institutes of Health"],"pagination":["839-51"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4138836"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["49(8)"],"pubmed_abstract":["A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of the antioxidant butylated hydroxytoluene and air-dried at room temperature. Transmethylation was accelerated by microwave irradiation in an explosion-proof multimode microwave reaction system. The chemical procedure was based on a one-step direct transmethylation procedure catalyzed by acetyl chloride. The short-term stability of PUFA in blood dried on filter paper and storage at room temperature was examined using venous blood. The recoveries ranged from 97 to 101 % for the cat"],"journal":["Lipids"],"pubmed_title":["Fast transmethylation of total lipids in dried blood by microwave irradiation and its application to a population study."],"pmcid":["PMC4138836"],"funding_grant_id":["ZIA AA000262-31"],"pubmed_authors":["Hanson JA","Strandjord SE","Salem NM","Dretsch MN","Lin YH","Haub MD","Hibbeln JR"],"additional_accession":[]},"is_claimable":false,"name":"Fast transmethylation of total lipids in dried blood by microwave irradiation and its application to a population study.","description":"A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of the antioxidant butylated hydroxytoluene and air-dried at room temperature. Transmethylation was accelerated by microwave irradiation in an explosion-proof multimode microwave reaction system. The chemical procedure was based on a one-step direct transmethylation procedure catalyzed by acetyl chloride. The short-term stability of PUFA in blood dried on filter paper and storage at room temperature was examined using venous blood. The recoveries ranged from 97 to 101 % for the cat","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Aug","modification":"2025-07-04T03:07:11.273Z","creation":"2025-07-04T03:07:11.273Z"},"accession":"S-EPMC4138836","cross_references":{"pubmed":["24986160"],"doi":["10.1007/s11745-014-3918-3"]}}