biostudies-literatureLin YHIntramural NIH HHSNational Institute on Alcohol Abuse and AlcoholismNational Institutes of Health839-51https://www.ebi.ac.uk/biostudies/studies/S-EPMC4138836biostudies-literatureUnknown49(8)A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of the antioxidant butylated hydroxytoluene and air-dried at room temperature. Transmethylation was accelerated by microwave irradiation in an explosion-proof multimode microwave reaction system. The chemical procedure was based on a one-step direct transmethylation procedure catalyzed by acetyl chloride. The short-term stability of PUFA in blood dried on filter paper and storage at room temperature was examined using venous blood. The recoveries ranged from 97 to 101 % for the catLipidsFast transmethylation of total lipids in dried blood by microwave irradiation and its application to a population study.PMC4138836ZIA AA000262-31Hanson JAStrandjord SESalem NMDretsch MNLin YHHaub MDHibbeln JRfalseFast transmethylation of total lipids in dried blood by microwave irradiation and its application to a population study.A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of the antioxidant butylated hydroxytoluene and air-dried at room temperature. Transmethylation was accelerated by microwave irradiation in an explosion-proof multimode microwave reaction system. The chemical procedure was based on a one-step direct transmethylation procedure catalyzed by acetyl chloride. The short-term stability of PUFA in blood dried on filter paper and storage at room temperature was examined using venous blood. The recoveries ranged from 97 to 101 % for the cat2014-01-01T00:00:00Z2014 Aug2025-07-04T03:07:11.273Z2025-07-04T03:07:11.273ZS-EPMC41388362498616010.1007/s11745-014-3918-3