{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(7)"],"submitter":["Liu YJ"],"pubmed_abstract":["<h4>Aim</h4>To elucidate the role of Rab23 in hepatocellular carcinoma (HCC) by assessing the expression of Rab23 in HCC tissue and in HCC cell lines.<h4>Methods</h4>Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of double-stranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescence-activated cell sorting.<h4>Results</h4>High cytoplasmic and nuclear expres"],"journal":["World journal of gastroenterology"],"pagination":["1010-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4146862"],"repository":["biostudies-literature"],"pubmed_title":["Rab23 is a potential biological target for treating hepatocellular carcinoma."],"pmcid":["PMC4146862"],"pubmed_authors":["Li W","Huang XH","Liu YJ","Wang Q","Zhen MC","Zhang HW","Huang SH","Xue L","Chen LZ"],"additional_accession":[]},"is_claimable":false,"name":"Rab23 is a potential biological target for treating hepatocellular carcinoma.","description":"<h4>Aim</h4>To elucidate the role of Rab23 in hepatocellular carcinoma (HCC) by assessing the expression of Rab23 in HCC tissue and in HCC cell lines.<h4>Methods</h4>Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of double-stranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescence-activated cell sorting.<h4>Results</h4>High cytoplasmic and nuclear expres","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Feb","modification":"2025-04-05T00:45:43.556Z","creation":"2019-06-06T13:13:10Z"},"accession":"S-EPMC4146862","cross_references":{"pubmed":["17373734"],"doi":["10.3748/wjg.v13.i7.1010"]}}