biostudies-literatureUnknown13(7)Liu YJ<h4>Aim</h4>To elucidate the role of Rab23 in hepatocellular carcinoma (HCC) by assessing the expression of Rab23 in HCC tissue and in HCC cell lines.<h4>Methods</h4>Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of double-stranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescence-activated cell sorting.<h4>Results</h4>High cytoplasmic and nuclear expresWorld journal of gastroenterology1010-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC4146862biostudies-literatureRab23 is a potential biological target for treating hepatocellular carcinoma.PMC4146862Li WHuang XHLiu YJWang QZhen MCZhang HWHuang SHXue LChen LZfalseRab23 is a potential biological target for treating hepatocellular carcinoma.<h4>Aim</h4>To elucidate the role of Rab23 in hepatocellular carcinoma (HCC) by assessing the expression of Rab23 in HCC tissue and in HCC cell lines.<h4>Methods</h4>Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of double-stranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescence-activated cell sorting.<h4>Results</h4>High cytoplasmic and nuclear expres2007-01-01T00:00:00Z2007 Feb2025-04-05T00:45:43.556Z2019-06-06T13:13:10ZS-EPMC41468621737373410.3748/wjg.v13.i7.1010