biostudies-literatureUnknown48(6)Cartelle MA clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (bla(CTX-M-32)) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysisAntimicrobial agents and chemotherapy2308-13https://www.ebi.ac.uk/biostudies/studies/S-EPMC415568biostudies-literatureHigh-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution.PMC415568Cartelle MMoure RBou Gdel Mar Tomas MMolina FVillanueva RfalseHigh-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution.A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (bla(CTX-M-32)) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysis2004-01-01T00:00:00Z2004 Jun2024-11-06T02:40:45.22Z2019-03-27T00:50:28ZS-EPMC4155681515524210.1128/AAC.48.6.2308-2313.200410.1128/aac.48.6.2308-2313.2004