biostudies-literatureMirza MKNCATS NIH HHSNCI NIH HHSAmerican Cancer Society Institutional Research GrantUniversity of Chicago Comprehensive Cancer Center Support Grant1091-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC4157130biostudies-literatureUnknown38(9)Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.Leukemia researchGenomic aberrations in myeloid sarcoma without blood or bone marrow involvement: characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays.PMC4157130UL1 TR000430IRG-58-004P30 CA14599P30 CA014599Joseph LEhninger GMirza MKRaca GStolzel FZophel KSukhanova MKuithan FOnel KReddy PLarson RAStock WfalseGenomic aberrations in myeloid sarcoma without blood or bone marrow involvement: characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays.Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.2014-01-01T00:00:00Z2014 Sep2025-04-19T08:55:31.522Z2019-03-27T01:35:21ZS-EPMC41571302508880810.1016/j.leukres.2014.05.004