{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2"],"submitter":["Liu Y"],"pubmed_abstract":["<h4>Background</h4>Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood AML.<h4>Procedure</h4>Ninety-nine children with newly diagnosed AML were included in this study. We developed a fluorescent dye SYTO-82 based high-resolution melting (HRM) curve analysis to detect FLT3 internal tandem duplication (FLT3-ITD), FLT3 tyrosine kinase domain (FLT3-TKD), and NPM1 mutations. MLL-PTD was screened by real-time quantitative PCR.<h4>Results</h4>The HRM methodology correlated well with gold s"],"journal":["Frontiers in pediatrics"],"pagination":["96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4158872"],"repository":["biostudies-literature"],"pubmed_title":["High-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia."],"pmcid":["PMC4158872"],"pubmed_authors":["Liu Y","Tang J","Chen J","Gaynon PS","Shen S","Sun W","Wakamatsu P","Xue H"],"additional_accession":[]},"is_claimable":false,"name":"High-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia.","description":"<h4>Background</h4>Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood AML.<h4>Procedure</h4>Ninety-nine children with newly diagnosed AML were included in this study. We developed a fluorescent dye SYTO-82 based high-resolution melting (HRM) curve analysis to detect FLT3 internal tandem duplication (FLT3-ITD), FLT3 tyrosine kinase domain (FLT3-TKD), and NPM1 mutations. MLL-PTD was screened by real-time quantitative PCR.<h4>Results</h4>The HRM methodology correlated well with gold s","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-04T11:20:34.13Z","creation":"2019-03-27T01:35:26Z"},"accession":"S-EPMC4158872","cross_references":{"pubmed":["25250304"],"doi":["10.3389/fped.2014.00096"]}}