biostudies-literatureUnknown2Liu Y<h4>Background</h4>Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood AML.<h4>Procedure</h4>Ninety-nine children with newly diagnosed AML were included in this study. We developed a fluorescent dye SYTO-82 based high-resolution melting (HRM) curve analysis to detect FLT3 internal tandem duplication (FLT3-ITD), FLT3 tyrosine kinase domain (FLT3-TKD), and NPM1 mutations. MLL-PTD was screened by real-time quantitative PCR.<h4>Results</h4>The HRM methodology correlated well with gold sFrontiers in pediatrics96https://www.ebi.ac.uk/biostudies/studies/S-EPMC4158872biostudies-literatureHigh-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia.PMC4158872Liu YTang JChen JGaynon PSShen SSun WWakamatsu PXue HfalseHigh-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia.<h4>Background</h4>Molecular genetic alterations with prognostic significance have been described in childhood acute myeloid leukemia (AML). The aim of this study was to establish cost-effective techniques to detect mutations of FMS-like tyrosine kinase 3 (FLT3), nucleophosmin 1 (NPM1), and a partial tandem duplication within the mixed-lineage leukemia (MLL-PTD) genes in childhood AML.<h4>Procedure</h4>Ninety-nine children with newly diagnosed AML were included in this study. We developed a fluorescent dye SYTO-82 based high-resolution melting (HRM) curve analysis to detect FLT3 internal tandem duplication (FLT3-ITD), FLT3 tyrosine kinase domain (FLT3-TKD), and NPM1 mutations. MLL-PTD was screened by real-time quantitative PCR.<h4>Results</h4>The HRM methodology correlated well with gold s2014-01-01T00:00:00Z20142025-04-04T11:20:34.13Z2019-03-27T01:35:26ZS-EPMC41588722525030410.3389/fped.2014.00096