{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jeong HJ"],"funding":["JSPS"],"pagination":["13285-97"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4168482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(7)"],"pubmed_abstract":["Antibody-based sensors have made outstanding contributions to the fields of molecular biology and biotechnology. Our group recently developed a novel powerful fluorescent immunosensor strategy named Quenchbody (Q-body), which has been applied to the detection of a range of antigens in a rapid, simple, and sensitive manner. However, there were some Q-bodies whose fluorescence response was limited, especially for detecting protein antigens. With the aim of improving this issue, here we made twelve types of Q-bodies incorporated with different number and position of TAMRA fluorophore in the single chain Fv of HyHEL-10, an anti-hen egg lysozyme antibody, as a model. By measuring the fluorescence intensity and its antigen dependency, it was revealed that VL-VH type Q-bodies labeled at a non-CDR"],"journal":["Sensors (Basel, Switzerland)"],"pubmed_title":["Strategy for making a superior Quenchbody to proteins: effect of the fluorophore position."],"pmcid":["PMC4168482"],"funding_grant_id":["Grant-in-Aid for Challenging Exploratory Research (25123456)","Grant-in-Aid for Scientific Research (B24360336)"],"pubmed_authors":["Jeong HJ","Ueda H"],"additional_accession":[]},"is_claimable":false,"name":"Strategy for making a superior Quenchbody to proteins: effect of the fluorophore position.","description":"Antibody-based sensors have made outstanding contributions to the fields of molecular biology and biotechnology. Our group recently developed a novel powerful fluorescent immunosensor strategy named Quenchbody (Q-body), which has been applied to the detection of a range of antigens in a rapid, simple, and sensitive manner. However, there were some Q-bodies whose fluorescence response was limited, especially for detecting protein antigens. With the aim of improving this issue, here we made twelve types of Q-bodies incorporated with different number and position of TAMRA fluorophore in the single chain Fv of HyHEL-10, an anti-hen egg lysozyme antibody, as a model. By measuring the fluorescence intensity and its antigen dependency, it was revealed that VL-VH type Q-bodies labeled at a non-CDR","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2025-04-03T22:43:03.042Z","creation":"2019-06-06T13:17:23Z"},"accession":"S-EPMC4168482","cross_references":{"pubmed":["25057138"],"doi":["10.3390/s140713285"]}}