biostudies-literatureJeong HJJSPS13285-97https://www.ebi.ac.uk/biostudies/studies/S-EPMC4168482biostudies-literatureUnknown14(7)Antibody-based sensors have made outstanding contributions to the fields of molecular biology and biotechnology. Our group recently developed a novel powerful fluorescent immunosensor strategy named Quenchbody (Q-body), which has been applied to the detection of a range of antigens in a rapid, simple, and sensitive manner. However, there were some Q-bodies whose fluorescence response was limited, especially for detecting protein antigens. With the aim of improving this issue, here we made twelve types of Q-bodies incorporated with different number and position of TAMRA fluorophore in the single chain Fv of HyHEL-10, an anti-hen egg lysozyme antibody, as a model. By measuring the fluorescence intensity and its antigen dependency, it was revealed that VL-VH type Q-bodies labeled at a non-CDRSensors (Basel, Switzerland)Strategy for making a superior Quenchbody to proteins: effect of the fluorophore position.PMC4168482Grant-in-Aid for Challenging Exploratory Research (25123456)Grant-in-Aid for Scientific Research (B24360336)Jeong HJUeda HfalseStrategy for making a superior Quenchbody to proteins: effect of the fluorophore position.Antibody-based sensors have made outstanding contributions to the fields of molecular biology and biotechnology. Our group recently developed a novel powerful fluorescent immunosensor strategy named Quenchbody (Q-body), which has been applied to the detection of a range of antigens in a rapid, simple, and sensitive manner. However, there were some Q-bodies whose fluorescence response was limited, especially for detecting protein antigens. With the aim of improving this issue, here we made twelve types of Q-bodies incorporated with different number and position of TAMRA fluorophore in the single chain Fv of HyHEL-10, an anti-hen egg lysozyme antibody, as a model. By measuring the fluorescence intensity and its antigen dependency, it was revealed that VL-VH type Q-bodies labeled at a non-CDR2014-01-01T00:00:00Z2014 Jul2025-04-03T22:43:03.042Z2019-06-06T13:17:23ZS-EPMC41684822505713810.3390/s140713285