{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Waldrip ZJ"],"funding":["NCRR NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["1207-11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4169012"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(9)"],"pubmed_abstract":["Any given chromosomal activity (e.g., transcription) is governed predominantly by the local epiproteome. However, defining local epiproteomes has been limited by a lack of effective technologies to isolate discrete sections of chromatin and to identify with precision specific proteins and histone posttranslational modifications (PTMs). We report the use of the Cas9 and guide RNA (gRNA) components of the CRISPR system for gRNA-directed purification of a discrete section of chromatin. Quantitative mass spectrometry provides for unambiguous identification of proteins and histone PTMs specifically associated with the enriched chromatin. This CRISPR-based Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) approach revealed changes in the local epiproteome of a promoter duri"],"journal":["Epigenetics"],"pubmed_title":["A CRISPR-based approach for proteomic analysis of a single genomic locus."],"pmcid":["PMC4169012"],"funding_grant_id":["R01GM081766","R01 GM098922","UL1 RR029884","P30 GM103450","R33 CA173264","P20GM103429","R01 GM081766","UL1RR029884","P20 GM103429","P30GM103450","R33CA173264","R01GM106024","R01GM098922","R01 GM106024"],"pubmed_authors":["Taverna SD","Byrum SD","Wahls WP","Gao J","Storey AJ","Byrd AK","Waldrip ZJ","Mackintosh SG","Raney KD","Tackett AJ"],"additional_accession":[]},"is_claimable":false,"name":"A CRISPR-based approach for proteomic analysis of a single genomic locus.","description":"Any given chromosomal activity (e.g., transcription) is governed predominantly by the local epiproteome. However, defining local epiproteomes has been limited by a lack of effective technologies to isolate discrete sections of chromatin and to identify with precision specific proteins and histone posttranslational modifications (PTMs). We report the use of the Cas9 and guide RNA (gRNA) components of the CRISPR system for gRNA-directed purification of a discrete section of chromatin. Quantitative mass spectrometry provides for unambiguous identification of proteins and histone PTMs specifically associated with the enriched chromatin. This CRISPR-based Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) approach revealed changes in the local epiproteome of a promoter duri","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Sep","modification":"2025-04-22T21:09:17.911Z","creation":"2019-03-27T01:35:58Z"},"accession":"S-EPMC4169012","cross_references":{"pubmed":["25147920"],"doi":["10.4161/epi.29919"]}}