biostudies-literatureValentino MDNEI NIH HHSNIAID NIH HHSe01729-14https://www.ebi.ac.uk/biostudies/studies/S-EPMC4173792biostudies-literatureUnknown5(5)<h4>Unlabelled</h4>Staphylococcus aureus is a leading cause of both community- and hospital-acquired infections that are increasingly antibiotic resistant. The emergence of S. aureus resistance to even last-line antibiotics heightens the need for the development of new drugs with novel targets. We generated a highly saturated transposon insertion mutant library in the genome of S. aureus and used Tn-seq analysis to probe the entire genome, with unprecedented resolution and sensitivity, for genes of importance in infection. We further identified genes contributing to fitness in various infected compartments (blood and ocular fluids) and compared them to genes required for growth in rich medium. This resulted in the identification of 426 genes that were important for S. aureus fitness duringmBioGenes contributing to Staphylococcus aureus fitness in abscess- and infection-related ecologies.PMC4173792T32-EY007145R21 AI107248AI107248AI083214P01 AI083214T32 EY007145Foulston LCamilli AGilmore MSVillet RAValentino MDKos VNSanta Maria JWalker SSadaka ALazinski DWHooper DCfalseGenes contributing to Staphylococcus aureus fitness in abscess- and infection-related ecologies.<h4>Unlabelled</h4>Staphylococcus aureus is a leading cause of both community- and hospital-acquired infections that are increasingly antibiotic resistant. The emergence of S. aureus resistance to even last-line antibiotics heightens the need for the development of new drugs with novel targets. We generated a highly saturated transposon insertion mutant library in the genome of S. aureus and used Tn-seq analysis to probe the entire genome, with unprecedented resolution and sensitivity, for genes of importance in infection. We further identified genes contributing to fitness in various infected compartments (blood and ocular fluids) and compared them to genes required for growth in rich medium. This resulted in the identification of 426 genes that were important for S. aureus fitness during2014-01-01T00:00:00Z2014 Sep2026-05-02T09:02:37.606Z2026-04-07T17:53:08.874ZS-EPMC41737922518232910.1128/mBio.01729-14