{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["44"],"submitter":["Wang C"],"pubmed_abstract":["Here we report the rescue of a recombinant porcine reproductive and respiratory syndrome virus (PRRSV) carrying an enhanced green fluorescent protein (EGFP) reporter gene as a separate transcription unit. A copy of the transcription regulatory sequence for ORF6 (TRS6) was inserted between the N protein and 3'-UTR to drive the transcription of the EGFP gene and yield a general purpose expression vector. Successful recovery of PRRSV was obtained using an RNA polymerase II promoter to drive transcription of the full-length virus genome, which was assembled in a bacterial artificial chromosome (BAC). The recombinant virus showed growth replication characteristics similar to those of the wild-type virus in the infected cells. In addition, the recombinant virus stably expressed EGFP for at least"],"journal":["Veterinary research"],"pagination":["104"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4176086"],"repository":["biostudies-literature"],"pubmed_title":["A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit."],"pmcid":["PMC4176086"],"pubmed_authors":["Kong N","Mu Y","Wang C","Ma Y","Gao J","Li Q","Xiao S","Sun Y","Zhou EM","Liang C","Dang L","Li Z","Zhao Q","Enjuanes L","Wang X","Zhang C","Huang B","Almazan F"],"additional_accession":[]},"is_claimable":false,"name":"A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit.","description":"Here we report the rescue of a recombinant porcine reproductive and respiratory syndrome virus (PRRSV) carrying an enhanced green fluorescent protein (EGFP) reporter gene as a separate transcription unit. A copy of the transcription regulatory sequence for ORF6 (TRS6) was inserted between the N protein and 3'-UTR to drive the transcription of the EGFP gene and yield a general purpose expression vector. Successful recovery of PRRSV was obtained using an RNA polymerase II promoter to drive transcription of the full-length virus genome, which was assembled in a bacterial artificial chromosome (BAC). The recombinant virus showed growth replication characteristics similar to those of the wild-type virus in the infected cells. In addition, the recombinant virus stably expressed EGFP for at least","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Oct","modification":"2026-04-08T02:47:25.497Z","creation":"2026-04-07T21:05:07Z"},"accession":"S-EPMC4176086","cross_references":{"pubmed":["24176053"],"doi":["10.1186/1297-9716-44-104"]}}