{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wan D"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["e3124"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4177753"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(9)"],"pubmed_abstract":["<h4>Background</h4>Parasite-specific IgE is thought to correlate with protection against Schistosoma mansoni infection or re-infection. Only a few molecular targets of the IgE response in S. mansoni infection have been characterised. A better insight into the basic mechanisms of anti-parasite immunity could be gained from a genome-wide characterisation of such S. mansoni allergens. This would have repercussions on our understanding of allergy and the development of safe and efficacious vaccinations against helminthic parasites.<h4>Methodology/principal findings</h4>A complete medium- to high-throughput amenable workflow, including important quality controls, is described, which enables the rapid translation of S. mansoni proteins using wheat germ lysate and subsequent assessment of potenti"],"journal":["PLoS neglected tropical diseases"],"pubmed_title":["Use of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins."],"pmcid":["PMC4177753"],"funding_grant_id":["967514"],"pubmed_authors":["Dunne DW","Al-Barwary N","Ali Ali E","Ludolf F","Stretton O","Doenhoff MJ","Alanine DG","Fitzsimmons CM","Wang X","Alcocer MJ","Nakamura R","Mari A","Wan D","Oliveira GC","Falcone FH"],"additional_accession":[]},"is_claimable":false,"name":"Use of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins.","description":"<h4>Background</h4>Parasite-specific IgE is thought to correlate with protection against Schistosoma mansoni infection or re-infection. Only a few molecular targets of the IgE response in S. mansoni infection have been characterised. A better insight into the basic mechanisms of anti-parasite immunity could be gained from a genome-wide characterisation of such S. mansoni allergens. This would have repercussions on our understanding of allergy and the development of safe and efficacious vaccinations against helminthic parasites.<h4>Methodology/principal findings</h4>A complete medium- to high-throughput amenable workflow, including important quality controls, is described, which enables the rapid translation of S. mansoni proteins using wheat germ lysate and subsequent assessment of potenti","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Sep","modification":"2025-04-29T10:37:40Z","creation":"2019-03-27T01:36:38Z"},"accession":"S-EPMC4177753","cross_references":{"pubmed":["25254513"],"doi":["10.1371/journal.pntd.0003124"]}}