biostudies-literatureWan DBiotechnology and Biological Sciences Research Councile3124https://www.ebi.ac.uk/biostudies/studies/S-EPMC4177753biostudies-literatureUnknown8(9)<h4>Background</h4>Parasite-specific IgE is thought to correlate with protection against Schistosoma mansoni infection or re-infection. Only a few molecular targets of the IgE response in S. mansoni infection have been characterised. A better insight into the basic mechanisms of anti-parasite immunity could be gained from a genome-wide characterisation of such S. mansoni allergens. This would have repercussions on our understanding of allergy and the development of safe and efficacious vaccinations against helminthic parasites.<h4>Methodology/principal findings</h4>A complete medium- to high-throughput amenable workflow, including important quality controls, is described, which enables the rapid translation of S. mansoni proteins using wheat germ lysate and subsequent assessment of potentiPLoS neglected tropical diseasesUse of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins.PMC4177753967514Dunne DWAl-Barwary NAli Ali ELudolf FStretton ODoenhoff MJAlanine DGFitzsimmons CMWang XAlcocer MJNakamura RMari AWan DOliveira GCFalcone FHfalseUse of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins.<h4>Background</h4>Parasite-specific IgE is thought to correlate with protection against Schistosoma mansoni infection or re-infection. Only a few molecular targets of the IgE response in S. mansoni infection have been characterised. A better insight into the basic mechanisms of anti-parasite immunity could be gained from a genome-wide characterisation of such S. mansoni allergens. This would have repercussions on our understanding of allergy and the development of safe and efficacious vaccinations against helminthic parasites.<h4>Methodology/principal findings</h4>A complete medium- to high-throughput amenable workflow, including important quality controls, is described, which enables the rapid translation of S. mansoni proteins using wheat germ lysate and subsequent assessment of potenti2014-01-01T00:00:00Z2014 Sep2025-04-29T10:37:40Z2019-03-27T01:36:38ZS-EPMC41777532525451310.1371/journal.pntd.0003124