biostudies-literatureHuang LBLRD VANIDDK NIH HHSNCI NIH HHS2303-15https://www.ebi.ac.uk/biostudies/studies/S-EPMC4178431biostudies-literatureUnknown25(10)Stanniocalcin-1 is an intracrine protein; it binds to the cell surface, is internalized to the mitochondria, and diminishes superoxide generation through induction of uncoupling proteins. In vitro, stanniocalcin-1 inhibits macrophages and preserves endothelial barrier function, and transgenic overexpression of stanniocalcin-1 in mice protects against ischemia-reperfusion kidney injury. We sought to determine the kidney phenotype after kidney endothelium-specific expression of stanniocalcin-1 small hairpin RNA (shRNA). We generated transgenic mice that express stanniocalcin-1 shRNA or scrambled shRNA upon removal of a floxed reporter (phosphoglycerate kinase-driven enhanced green fluorescent protein) and used ultrasound microbubbles to deliver tyrosine kinase receptor-2 promoter-driven Cre Journal of the American Society of Nephrology : JASNAKI after conditional and kidney-specific knockdown of stanniocalcin-1.PMC4178431R01 CA116097R01-DK080306P30-CA125123T32-DK062706T32 DK062706R01 DK080306I01 BX002006P30 CA125123R01 CA122623Belousova TJu HPan JSLu LNuotio-Antar AHuang LDu JSheikh-Hamad DTruong LDZhang PfalseAKI after conditional and kidney-specific knockdown of stanniocalcin-1.Stanniocalcin-1 is an intracrine protein; it binds to the cell surface, is internalized to the mitochondria, and diminishes superoxide generation through induction of uncoupling proteins. In vitro, stanniocalcin-1 inhibits macrophages and preserves endothelial barrier function, and transgenic overexpression of stanniocalcin-1 in mice protects against ischemia-reperfusion kidney injury. We sought to determine the kidney phenotype after kidney endothelium-specific expression of stanniocalcin-1 small hairpin RNA (shRNA). We generated transgenic mice that express stanniocalcin-1 shRNA or scrambled shRNA upon removal of a floxed reporter (phosphoglycerate kinase-driven enhanced green fluorescent protein) and used ultrasound microbubbles to deliver tyrosine kinase receptor-2 promoter-driven Cre 2014-01-01T00:00:00Z2014 Oct2026-05-02T00:16:04.947Z2019-03-27T01:36:40ZS-EPMC41784312470087810.1681/ASN.201307069010.1681/asn.2013070690