biostudies-literatureGong DNIDCR NIH HHSNIAID NIH HHSNCI NIH HHS11369-82https://www.ebi.ac.uk/biostudies/studies/S-EPMC4178812biostudies-literatureUnknown88(19)<h4>Unlabelled</h4>Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with several human malignances. As saliva is likely the major vehicle for KSHV transmission, we studied in vitro KSHV infection of oral epithelial cells. Through infection of two types of oral epithelial cells, normal human oral keratinocytes (NHOKs) and papilloma-immortalized human oral keratinocyte (HOK16B) cells, we found that KSHV can undergo robust lytic replication in oral epithelial cells. By employing de novo lytic infection of HOK16B cells, we studied the functions of two previously uncharacterized genes, ORF18 and ORF30, during the KSHV lytic cycle. For this purpose, an ORF18-deficient virus and an ORF30-deficient virus were generated using a mutagenesis strategy based on bacterial artificial chromosoJournal of virologyKaposi's sarcoma-associated herpesvirus ORF18 and ORF30 are essential for late gene expression during lytic replication.PMC4178812P01 CA180779P30 CA016042AI078133R01 CA115284R01 DE015752R01 CA091791R21 AI078133DE15752CA91791Park NHXie YTong LWu NCDu YBrulois KFLuan HGong DWang CYFeng JJung JUSun RKang MKWu TTfalseKaposi's sarcoma-associated herpesvirus ORF18 and ORF30 are essential for late gene expression during lytic replication.<h4>Unlabelled</h4>Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with several human malignances. As saliva is likely the major vehicle for KSHV transmission, we studied in vitro KSHV infection of oral epithelial cells. Through infection of two types of oral epithelial cells, normal human oral keratinocytes (NHOKs) and papilloma-immortalized human oral keratinocyte (HOK16B) cells, we found that KSHV can undergo robust lytic replication in oral epithelial cells. By employing de novo lytic infection of HOK16B cells, we studied the functions of two previously uncharacterized genes, ORF18 and ORF30, during the KSHV lytic cycle. For this purpose, an ORF18-deficient virus and an ORF30-deficient virus were generated using a mutagenesis strategy based on bacterial artificial chromoso2014-01-01T00:00:00Z2014 Oct2025-04-22T20:42:05.775Z2019-03-27T01:36:41ZS-EPMC41788122505689610.1128/jvi.00793-1410.1128/JVI.00793-14