biostudies-literatureUnknown1(2)Mikaelian ISerum cardiac troponins are the key biomarkers of myocardial necrosis in humans and in preclinical species. The use of ultrasensitive assays for serum cardiac troponin I (cTnI) as a biomarker in safety studies is hampered by interindividual differences. In this study, we investigated the effect of handling procedures on serum cTnI and explored modeling and simulation approaches to mitigate the impact of these interindividual differences. Femoral-catheterized male Crl:WI(Han) rats (n = 16/group) were left undisturbed in their cages with no handling; subjected to 5 min of isoflurane/O2 anesthesia (A); or placed into a rodent restrainer followed by simulated tail vein injection (RR). Serum cTnI concentrations were assessed over a 24-h period using an ultrasensitive assay, and the study was rePharmacology research & perspectivese00011https://www.ebi.ac.uk/biostudies/studies/S-EPMC4186429biostudies-literatureDifferential analysis of transient increases of serum cTnI in response to handling in rats.PMC4186429Nicklaus RGeng WMould DRDunn MEHirkaler GReddy MMikaelian IColuccio DSinger TfalseDifferential analysis of transient increases of serum cTnI in response to handling in rats.Serum cardiac troponins are the key biomarkers of myocardial necrosis in humans and in preclinical species. The use of ultrasensitive assays for serum cardiac troponin I (cTnI) as a biomarker in safety studies is hampered by interindividual differences. In this study, we investigated the effect of handling procedures on serum cTnI and explored modeling and simulation approaches to mitigate the impact of these interindividual differences. Femoral-catheterized male Crl:WI(Han) rats (n = 16/group) were left undisturbed in their cages with no handling; subjected to 5 min of isoflurane/O2 anesthesia (A); or placed into a rodent restrainer followed by simulated tail vein injection (RR). Serum cTnI concentrations were assessed over a 24-h period using an ultrasensitive assay, and the study was re2013-01-01T00:00:00Z2013 Dec2025-05-31T23:03:02.051Z2025-05-31T23:03:02.051ZS-EPMC41864292550556610.1002/prp2.11