{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["196(19)"],"submitter":["Steiner EM"],"pubmed_abstract":["Mycobacterium tuberculosis is dependent on cysteine biosynthesis, and reduced sulfur compounds such as mycothiol synthesized from cysteine serve in first-line defense mechanisms against oxidative stress imposed by macrophages. Two biosynthetic routes to l-cysteine, each with its own specific cysteine synthase (CysK1 and CysM), have been described in M. tuberculosis, but the function of a third putative sulfhydrylase in this pathogen, CysK2, has remained elusive. We present biochemical and biophysical evidence that CysK2 is an S-sulfocysteine synthase, utilizing O-phosphoserine (OPS) and thiosulfate as substrates. The enzyme uses a mechanism via a central aminoacrylate intermediate that is similar to that of other members of this pyridoxal phosphate-dependent enzyme family. The apparent sec"],"journal":["Journal of bacteriology"],"pagination":["3410-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4187678"],"repository":["biostudies-literature"],"pubmed_title":["CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase."],"pmcid":["PMC4187678"],"pubmed_authors":["Schneider G","Schnell R","Lossl P","Vilaplana F","Both D","Steiner EM"],"additional_accession":[]},"is_claimable":false,"name":"CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase.","description":"Mycobacterium tuberculosis is dependent on cysteine biosynthesis, and reduced sulfur compounds such as mycothiol synthesized from cysteine serve in first-line defense mechanisms against oxidative stress imposed by macrophages. Two biosynthetic routes to l-cysteine, each with its own specific cysteine synthase (CysK1 and CysM), have been described in M. tuberculosis, but the function of a third putative sulfhydrylase in this pathogen, CysK2, has remained elusive. We present biochemical and biophysical evidence that CysK2 is an S-sulfocysteine synthase, utilizing O-phosphoserine (OPS) and thiosulfate as substrates. The enzyme uses a mechanism via a central aminoacrylate intermediate that is similar to that of other members of this pyridoxal phosphate-dependent enzyme family. The apparent sec","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Oct","modification":"2025-05-31T23:04:03.146Z","creation":"2025-05-31T23:04:03.146Z"},"accession":"S-EPMC4187678","cross_references":{"pubmed":["25022854"],"doi":["10.1128/JB.01851-14","10.1128/jb.01851-14"]}}