biostudies-literatureUnknown114(6)Buffetto F<h4>Background and aims</h4>Rhamnogalacturonan II (RGII) is a structurally complex pectic sub-domain composed of more than 12 different sugars and 20 different linkages distributed in five side chains along a homogalacturonan backbone. Although RGII has long been described as highly conserved over plant evolution, recent studies have revealed variations in the structure of the polysaccharide. This study examines the fine structure variability of RGII in wine, focusing on the side chains A and B obtained after sequential mild acid hydrolysis. Specifically, this study aims to differentiate intrinsic structural variations in these RGII side chains from structural variations due to acid hydrolysis.<h4>Methods</h4>RGII from wine (Vitis vinifera Merlot) was sequentially hydrolysed with trifluoroAnnals of botany1327-37https://www.ebi.ac.uk/biostudies/studies/S-EPMC4195555biostudies-literatureRecovery and fine structure variability of RGII sub-domains in wine (Vitis vinifera Merlot).PMC4195555Zhang XJGuillon FRopartz DGilbert HJRalet MCBuffetto FfalseRecovery and fine structure variability of RGII sub-domains in wine (Vitis vinifera Merlot).<h4>Background and aims</h4>Rhamnogalacturonan II (RGII) is a structurally complex pectic sub-domain composed of more than 12 different sugars and 20 different linkages distributed in five side chains along a homogalacturonan backbone. Although RGII has long been described as highly conserved over plant evolution, recent studies have revealed variations in the structure of the polysaccharide. This study examines the fine structure variability of RGII in wine, focusing on the side chains A and B obtained after sequential mild acid hydrolysis. Specifically, this study aims to differentiate intrinsic structural variations in these RGII side chains from structural variations due to acid hydrolysis.<h4>Methods</h4>RGII from wine (Vitis vinifera Merlot) was sequentially hydrolysed with trifluoro2014-01-01T00:00:00Z2014 Oct2025-04-26T20:03:45.224Z2019-03-27T01:37:30ZS-EPMC41955552490868010.1093/aob/mcu097