biostudies-literatureHegemann BAustrian Science Fund FWFNIEHS NIH HHSNCI NIH HHSNIGMS NIH HHSrs12https://www.ebi.ac.uk/biostudies/studies/S-EPMC4206221biostudies-literatureUnknown4(198)Progression through mitosis depends on a large number of protein complexes that regulate the major structural and physiological changes necessary for faithful chromosome segregation. Most, if not all, of the mitotic processes are regulated by a set of mitotic protein kinases that control protein activity by phosphorylation. Although many mitotic phosphorylation events have been identified in proteome-scale mass spectrometry studies, information on how these phosphorylation sites are distributed within mitotic protein complexes and which kinases generate these phosphorylation sites is largely lacking. We used systematic protein-affinity purification combined with mass spectrometry to identify 1818 phosphorylation sites in more than 100 mitotic protein complexes. In many complexes, the phospScience signalingSystematic phosphorylation analysis of human mitotic protein complexes.PMC4206221CA-112967P50 GM068762GM-60594R01 GM060594GM-68762ES-015339U54 CA112967F 3407R01 ES015339Hudecz OMazanek MHeriche JKRameseder JHyman AAYaffe MBPoser ILiu SMechtler KLenart PKraut NNovatchkova MHegemann BSykora MMHutchins JRPeters JMfalseSystematic phosphorylation analysis of human mitotic protein complexes.Progression through mitosis depends on a large number of protein complexes that regulate the major structural and physiological changes necessary for faithful chromosome segregation. Most, if not all, of the mitotic processes are regulated by a set of mitotic protein kinases that control protein activity by phosphorylation. Although many mitotic phosphorylation events have been identified in proteome-scale mass spectrometry studies, information on how these phosphorylation sites are distributed within mitotic protein complexes and which kinases generate these phosphorylation sites is largely lacking. We used systematic protein-affinity purification combined with mass spectrometry to identify 1818 phosphorylation sites in more than 100 mitotic protein complexes. In many complexes, the phosp2011-01-01T00:00:00Z2011 Nov2025-07-11T03:04:02.795Z2025-07-11T03:04:02.795ZS-EPMC42062212206746010.1126/scisignal.2001993