biostudies-literatureChoi MNIMH NIH HHSe109253https://www.ebi.ac.uk/biostudies/studies/S-EPMC4207676biostudies-literatureUnknown9(10)Functional consequences to which vertebrate GATA transcription factors contribute in the adult brain remain largely an open question. The present study examines how human GATA-1 and GATA-2 (hGATA-1 and hGATA-2) are linked to neuronal differentiation and depressive behaviors in rats. We investigated the effects of adeno-associated viral expression of hGATA-1 and hGATA-2 (AAV-hGATA1 and AAV-hGATA2) in the dentate gyrus (DG) of the dorsal hippocampus on dendrite branching and spine number. We also examined the influence of AAV-hGATA1 and AAV-hGATA2 infusions into the dorsal hippocampus on rodent behavior in models of depression. Viral expression of hGATA-1 and hGATA-2 cDNA in rat hippocampal neurons impaired dendritic outgrowth and spine formation. Moreover, viral-mediated expression of hGATAPloS oneOverexpression of human GATA-1 and GATA-2 interferes with spine formation and produces depressive behavior in rats.PMC4207676R01 MH093897R01 MH105910Ko SYChae SYKang HJLee SHDuman RSChoi MWang SEKim YSSon HfalseOverexpression of human GATA-1 and GATA-2 interferes with spine formation and produces depressive behavior in rats.Functional consequences to which vertebrate GATA transcription factors contribute in the adult brain remain largely an open question. The present study examines how human GATA-1 and GATA-2 (hGATA-1 and hGATA-2) are linked to neuronal differentiation and depressive behaviors in rats. We investigated the effects of adeno-associated viral expression of hGATA-1 and hGATA-2 (AAV-hGATA1 and AAV-hGATA2) in the dentate gyrus (DG) of the dorsal hippocampus on dendrite branching and spine number. We also examined the influence of AAV-hGATA1 and AAV-hGATA2 infusions into the dorsal hippocampus on rodent behavior in models of depression. Viral expression of hGATA-1 and hGATA-2 cDNA in rat hippocampal neurons impaired dendritic outgrowth and spine formation. Moreover, viral-mediated expression of hGATA2014-01-01T00:00:00Z20142025-04-18T16:19:15.969Z2019-03-26T23:28:51ZS-EPMC42076762534077210.1371/journal.pone.0109253