biostudies-literatureCarroll VAmerican Heart AssociationNIBIB NIH HHSHoward Hughes Medical InstituteU.S. Department of Health and Human ServicesNCI NIH HHSNational Institute of General Medical SciencesNIGMS NIH HHS14742-5https://www.ebi.ac.uk/biostudies/studies/S-EPMC4210116biostudies-literatureUnknown136(42)Reactive oxygen species (ROS) play important roles in the development and progression of cancer and other diseases, motivating the development of translatable technologies for biological ROS imaging. Here we report Peroxy-Caged-[(18)F]Fluorodeoxy thymidine-1 (PC-FLT-1), an oxidatively immolative positron emission tomography (PET) probe for H2O2 detection. PC-FLT-1 reacts with H2O2 to generate [(18)F]FLT, allowing its peroxide-dependent uptake and retention in proliferating cells. The relative uptake of PC-FLT-1 was evaluated using H2O2-treated UOK262 renal carcinoma cells and a paraquat-induced oxidative stress cell model, demonstrating ROS-dependent tracer accumulation. The data suggest that PC-FLT-1 possesses promising characteristics for translatable ROS detection and provide a general Journal of the American Chemical SocietyA boronate-caged [¹⁸F]FLT probe for hydrogen peroxide detection using positron emission tomography.PMC4210116P41 EB013598RO1 GM79465R01 GM079465R01 GM79465R01 CA166766R00 EB014328RO1 CA166766Blecha JKeshari KVanBrocklin HWilson DChang CJCarroll VMichel BWfalseA boronate-caged [¹⁸F]FLT probe for hydrogen peroxide detection using positron emission tomography.Reactive oxygen species (ROS) play important roles in the development and progression of cancer and other diseases, motivating the development of translatable technologies for biological ROS imaging. Here we report Peroxy-Caged-[(18)F]Fluorodeoxy thymidine-1 (PC-FLT-1), an oxidatively immolative positron emission tomography (PET) probe for H2O2 detection. PC-FLT-1 reacts with H2O2 to generate [(18)F]FLT, allowing its peroxide-dependent uptake and retention in proliferating cells. The relative uptake of PC-FLT-1 was evaluated using H2O2-treated UOK262 renal carcinoma cells and a paraquat-induced oxidative stress cell model, demonstrating ROS-dependent tracer accumulation. The data suggest that PC-FLT-1 possesses promising characteristics for translatable ROS detection and provide a general 2014-01-01T00:00:00Z2014 Oct2025-04-22T21:09:58.716Z2019-03-27T01:38:23ZS-EPMC42101162531036910.1021/ja509198w