{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Merritt KK"],"pubmed_abstract":["<h4>Background</h4>Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis. However, the low information density of DNA built from just four nucleotide \"letters\", the presence of strong (G:C) and weak (A:T) nucleobase pairs, the non-canonical folded structures that compete with Watson-Crick pairing, and other features intrinsic to natural DNA, generally prevent the autonomous assembly of short single-stranded oligonucleotides greater than a dozen or so.<h4>Results</h4>We describe a new strategy to autonomously assemble L-DNA constructs from fragments of synthetic single-stranded DNA. This strategy uses an artificially expa"],"journal":["Beilstein journal of organic chemistry"],"pagination":["2348-60"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4222377"],"repository":["biostudies-literature"],"pubmed_title":["Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance."],"pmcid":["PMC4222377"],"pubmed_authors":["Rowold DJ","Merritt KK","Hutter D","Matsuura MF","Bradley KM","Benner SA"],"additional_accession":[]},"is_claimable":false,"name":"Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance.","description":"<h4>Background</h4>Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis. However, the low information density of DNA built from just four nucleotide \"letters\", the presence of strong (G:C) and weak (A:T) nucleobase pairs, the non-canonical folded structures that compete with Watson-Crick pairing, and other features intrinsic to natural DNA, generally prevent the autonomous assembly of short single-stranded oligonucleotides greater than a dozen or so.<h4>Results</h4>We describe a new strategy to autonomously assemble L-DNA constructs from fragments of synthetic single-stranded DNA. This strategy uses an artificially expa","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-18T16:18:27.209Z","creation":"2019-03-26T22:30:51Z"},"accession":"S-EPMC4222377","cross_references":{"pubmed":["25383105"],"doi":["10.3762/bjoc.10.245"]}}