{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bakshi S"],"funding":["National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["871-87"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4227943"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["94(4)"],"pubmed_abstract":["Previously observed effects of rifampicin and chloramphenicol indicate that transcription and translation activity strongly affect the coarse spatial organization of the bacterial cytoplasm. Single-cell, time-resolved, quantitative imaging of chromosome and ribosome spatial distributions and ribosome diffusion in live Escherichia coli provides insight into the underlying mechanisms. Monte Carlo simulations of model DNA-ribosome mixtures support a novel nucleoid-ribosome mixing hypothesis. In normal conditions, 70S-polysomes and the chromosomal DNA segregate, while 30S and 50S ribosomal subunits are able to penetrate the nucleoids. Growth conditions and drug treatments determine the partitioning of ribosomes into 70S-polysomes versus free 30S and 50S subunits. Entropic and excluded volume e"],"journal":["Molecular microbiology"],"pubmed_title":["Time-dependent effects of transcription- and translation-halting drugs on the spatial distributions of the Escherichia coli chromosome and ribosomes."],"pmcid":["PMC4227943"],"funding_grant_id":["R01 GM094510","R01-GM093265","CHE-1213860","R01 GM093265","R01-GM094510"],"pubmed_authors":["Weisshaar JC","Choi H","Bakshi S","Mondal J"],"additional_accession":[]},"is_claimable":false,"name":"Time-dependent effects of transcription- and translation-halting drugs on the spatial distributions of the Escherichia coli chromosome and ribosomes.","description":"Previously observed effects of rifampicin and chloramphenicol indicate that transcription and translation activity strongly affect the coarse spatial organization of the bacterial cytoplasm. Single-cell, time-resolved, quantitative imaging of chromosome and ribosome spatial distributions and ribosome diffusion in live Escherichia coli provides insight into the underlying mechanisms. Monte Carlo simulations of model DNA-ribosome mixtures support a novel nucleoid-ribosome mixing hypothesis. In normal conditions, 70S-polysomes and the chromosomal DNA segregate, while 30S and 50S ribosomal subunits are able to penetrate the nucleoids. Growth conditions and drug treatments determine the partitioning of ribosomes into 70S-polysomes versus free 30S and 50S subunits. Entropic and excluded volume e","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Nov","modification":"2025-07-02T03:04:27.658Z","creation":"2025-07-02T03:04:27.658Z"},"accession":"S-EPMC4227943","cross_references":{"pubmed":["25250841"],"doi":["10.1111/mmi.12805"]}}