{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Havre PA"],"pubmed_abstract":["<h4>Background</h4>CD26/dipeptidyl peptidase IV (DPPIV) is a multifunctional membrane protein with a key role in T-cell biology and also serves as a marker of aggressive cancers, including T-cell malignancies.<h4>Methods</h4>Versican expression was measured by real-time RT-PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was performed using transduction-ready viral particles. The effect of versican depletion on surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation. CD44 secretion/cleavage and ERK (1/2) activation was followed by Western blotting. Collagenase I activity was measured by a live cell assay and in vesicles using a liquid-phase assay. Adhesion to collagen I was quantified by an MTS assay.<h4>Results</h4>Versican exp"],"journal":["BMC cancer"],"pagination":["517"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4228418"],"repository":["biostudies-literature"],"pubmed_title":["CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion."],"pmcid":["PMC4228418"],"pubmed_authors":["Havre PA","Ohnuma K","Morimoto C","Iwata S","Dang LH","Dang NH"],"additional_accession":[]},"is_claimable":false,"name":"CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.","description":"<h4>Background</h4>CD26/dipeptidyl peptidase IV (DPPIV) is a multifunctional membrane protein with a key role in T-cell biology and also serves as a marker of aggressive cancers, including T-cell malignancies.<h4>Methods</h4>Versican expression was measured by real-time RT-PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was performed using transduction-ready viral particles. The effect of versican depletion on surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation. CD44 secretion/cleavage and ERK (1/2) activation was followed by Western blotting. Collagenase I activity was measured by a live cell assay and in vesicles using a liquid-phase assay. Adhesion to collagen I was quantified by an MTS assay.<h4>Results</h4>Versican exp","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Nov","modification":"2025-04-22T18:40:22.773Z","creation":"2021-02-21T06:04:24Z"},"accession":"S-EPMC4228418","cross_references":{"pubmed":["24180670"],"doi":["10.1186/1471-2407-13-517"]}}