biostudies-literatureZhou JAmerican Heart AssociationNatural Science Foundation of ChinaNHLBI NIH HHSPriority Academic Program Development of Jiangsu Higher Education InstitutionsTemple University School of MedicineNIH1890-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC4229395biostudies-literatureUnknown12(11)<h4>Background</h4>ERp57 is required for platelet function; however, whether ERp57 contributes to fibrin generation is unknown.<h4>Methods and results</h4>Using an inhibitory anti-ERp57 antibody (mAb1), Pf4-Cre/ERp57(fl/fl) mice, Tie2-Cre/ERp57(fl/fl) mice, and mutants of ERp57, we analyzed the function of ERp57 in laser-induced thrombosis. Fibrin deposition was decreased in Pf4-Cre/ERp57(fl/fl) mice, consistent with a role for platelet ERp57 in fibrin generation. Fibrin deposition was further decreased with infusion of mAb1 and in Tie2-Cre/ERp57(fl/fl) mice, consistent with endothelial cells also contributing to fibrin deposition. Infusion of eptibifatide inhibited platelet and fibrin deposition, confirming a role for platelets in fibrin deposition. Infusion of recombinant ERp57 correctedJournal of thrombosis and haemostasis : JTHThe disulfide isomerase ERp57 is required for fibrin deposition in vivo.PMC4229395P01 HL110860P01 HL06419031201058U01 HL0996561R01HL118526U01 HL09999381270592R01 HL118526Hayes VMRauova LPoncz MEssex DWWu YZhou JWang LfalseThe disulfide isomerase ERp57 is required for fibrin deposition in vivo.<h4>Background</h4>ERp57 is required for platelet function; however, whether ERp57 contributes to fibrin generation is unknown.<h4>Methods and results</h4>Using an inhibitory anti-ERp57 antibody (mAb1), Pf4-Cre/ERp57(fl/fl) mice, Tie2-Cre/ERp57(fl/fl) mice, and mutants of ERp57, we analyzed the function of ERp57 in laser-induced thrombosis. Fibrin deposition was decreased in Pf4-Cre/ERp57(fl/fl) mice, consistent with a role for platelet ERp57 in fibrin generation. Fibrin deposition was further decreased with infusion of mAb1 and in Tie2-Cre/ERp57(fl/fl) mice, consistent with endothelial cells also contributing to fibrin deposition. Infusion of eptibifatide inhibited platelet and fibrin deposition, confirming a role for platelets in fibrin deposition. Infusion of recombinant ERp57 corrected2014-01-01T00:00:00Z2014 Nov2025-07-02T03:04:29.758Z2025-07-02T03:04:29.758ZS-EPMC42293952515652110.1111/jth.12709