{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2"],"submitter":["Othman RT"],"pubmed_abstract":["<h4>Introduction</h4>Medulloblastoma (MB) is the most common malignant paediatric brain tumour. Recurrence and progression of disease occurs in 15-20% of standard risk and 30-40% of high risk patients. We analysed whether circumvention of chemoresistance pathways (drug export, DNA repair and apoptotic inhibition) can restore chemotherapeutic efficacy in a panel of MB cell lines.<h4>Results</h4>We demonstrate, by immunohistochemistry in patient tissue microarrays, that ABCB1 is expressed in 43% of tumours and is significantly associated with high-risk. We show that ABCB1, O6-methylguanine-DNA-methyltransferase (MGMT) and BCL2 family members are differentially expressed (by quantitative reverse transcription polymerase chain reaction, Western blotting and flow cytometry) in MB cell lines. Ba"],"journal":["Acta neuropathologica communications"],"pagination":["57"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4229867"],"repository":["biostudies-literature"],"pubmed_title":["Overcoming multiple drug resistance mechanisms in medulloblastoma."],"pmcid":["PMC4229867"],"pubmed_authors":["Kimishi I","Bradshaw TD","Othman RT","Kerr ID","Grundy RG","Pfister SM","Storer LC","Korshunov A","Coyle B"],"additional_accession":[]},"is_claimable":false,"name":"Overcoming multiple drug resistance mechanisms in medulloblastoma.","description":"<h4>Introduction</h4>Medulloblastoma (MB) is the most common malignant paediatric brain tumour. Recurrence and progression of disease occurs in 15-20% of standard risk and 30-40% of high risk patients. We analysed whether circumvention of chemoresistance pathways (drug export, DNA repair and apoptotic inhibition) can restore chemotherapeutic efficacy in a panel of MB cell lines.<h4>Results</h4>We demonstrate, by immunohistochemistry in patient tissue microarrays, that ABCB1 is expressed in 43% of tumours and is significantly associated with high-risk. We show that ABCB1, O6-methylguanine-DNA-methyltransferase (MGMT) and BCL2 family members are differentially expressed (by quantitative reverse transcription polymerase chain reaction, Western blotting and flow cytometry) in MB cell lines. Ba","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 May","modification":"2025-04-05T00:22:23.447Z","creation":"2019-03-27T01:39:28Z"},"accession":"S-EPMC4229867","cross_references":{"pubmed":["24887326"],"doi":["10.1186/2051-5960-2-57"]}}