biostudies-literatureUnknown2Othman RT<h4>Introduction</h4>Medulloblastoma (MB) is the most common malignant paediatric brain tumour. Recurrence and progression of disease occurs in 15-20% of standard risk and 30-40% of high risk patients. We analysed whether circumvention of chemoresistance pathways (drug export, DNA repair and apoptotic inhibition) can restore chemotherapeutic efficacy in a panel of MB cell lines.<h4>Results</h4>We demonstrate, by immunohistochemistry in patient tissue microarrays, that ABCB1 is expressed in 43% of tumours and is significantly associated with high-risk. We show that ABCB1, O6-methylguanine-DNA-methyltransferase (MGMT) and BCL2 family members are differentially expressed (by quantitative reverse transcription polymerase chain reaction, Western blotting and flow cytometry) in MB cell lines. BaActa neuropathologica communications57https://www.ebi.ac.uk/biostudies/studies/S-EPMC4229867biostudies-literatureOvercoming multiple drug resistance mechanisms in medulloblastoma.PMC4229867Kimishi IBradshaw TDOthman RTKerr IDGrundy RGPfister SMStorer LCKorshunov ACoyle BfalseOvercoming multiple drug resistance mechanisms in medulloblastoma.<h4>Introduction</h4>Medulloblastoma (MB) is the most common malignant paediatric brain tumour. Recurrence and progression of disease occurs in 15-20% of standard risk and 30-40% of high risk patients. We analysed whether circumvention of chemoresistance pathways (drug export, DNA repair and apoptotic inhibition) can restore chemotherapeutic efficacy in a panel of MB cell lines.<h4>Results</h4>We demonstrate, by immunohistochemistry in patient tissue microarrays, that ABCB1 is expressed in 43% of tumours and is significantly associated with high-risk. We show that ABCB1, O6-methylguanine-DNA-methyltransferase (MGMT) and BCL2 family members are differentially expressed (by quantitative reverse transcription polymerase chain reaction, Western blotting and flow cytometry) in MB cell lines. Ba2014-01-01T00:00:00Z2014 May2025-04-05T00:22:23.447Z2019-03-27T01:39:28ZS-EPMC42298672488732610.1186/2051-5960-2-57