{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jin S"],"funding":["NHLBI NIH HHS","NIH","NIGMS NIH HHS"],"pagination":["1274-85"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4247799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(9)"],"pubmed_abstract":["The γ-tocopherol methyltransferase (γ-TMT) is an important enzyme regulating synthesis of four tocopherols (α, γ, β and δ). In this report, we investigated the role of γ-TMT in regulating abiotic stress within chloroplasts. The At γ-tmt overexpressed via the tobacco chloroplast genome accumulated up to 7.7% of the total leaf protein, resulting in massive proliferation of the inner envelope membrane (IEM, up to eight layers). Such high-level expression of γ-TMT converted most of γ-tocopherol to α-tocopherol in transplastomic seeds (~10-fold higher) in the absence of abiotic stress. When grown in 400 mm NaCl, α-tocopherol content in transplastomic TMT leaves increased up to 8.2-fold and 2.4-fold higher than wild-type leaves. Likewise, under heavy metal stress, α-tocopherol content in the TMT"],"journal":["Plant biotechnology journal"],"pubmed_title":["Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species."],"pmcid":["PMC4247799"],"funding_grant_id":["R01 HL 109442","R01 GM063879","R01 HL 107904","R01 GM 63879","R01 HL109442","R01 HL107904"],"pubmed_authors":["Jin S","Daniell H"],"additional_accession":[]},"is_claimable":false,"name":"Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species.","description":"The γ-tocopherol methyltransferase (γ-TMT) is an important enzyme regulating synthesis of four tocopherols (α, γ, β and δ). In this report, we investigated the role of γ-TMT in regulating abiotic stress within chloroplasts. The At γ-tmt overexpressed via the tobacco chloroplast genome accumulated up to 7.7% of the total leaf protein, resulting in massive proliferation of the inner envelope membrane (IEM, up to eight layers). Such high-level expression of γ-TMT converted most of γ-tocopherol to α-tocopherol in transplastomic seeds (~10-fold higher) in the absence of abiotic stress. When grown in 400 mm NaCl, α-tocopherol content in transplastomic TMT leaves increased up to 8.2-fold and 2.4-fold higher than wild-type leaves. Likewise, under heavy metal stress, α-tocopherol content in the TMT","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Dec","modification":"2025-04-19T23:09:45.197Z","creation":"2019-03-27T01:40:29Z"},"accession":"S-EPMC4247799","cross_references":{"pubmed":["25051898"],"doi":["10.1111/pbi.12224"]}}