biostudies-literatureUnknown20(44)Yoon YSThe evaluation of therapeutic efficacy is necessary to predict the outcome of patients with metastatic colorectal cancer (CRC). In these patients, there is a critical need for predictive chemosensitivity assays and biomarkers to optimize efficacy and minimize toxicity. The introduction of targeted agents has improved the progression-free survival and overall survival of patients with metastatic disease. However, approximately 50% of patients do not show a positive response to chemotherapy and the selection of patients likely to respond to a specific regimen remains challenging. Cell culture-based chemosensitivity tests use autologous viable tumor cells to evaluate susceptibility to specific agents in vitro and predict their direct effects. Adenosine triphosphate-based assays and methyl thiWorld journal of gastroenterology16398-408https://www.ebi.ac.uk/biostudies/studies/S-EPMC4248183biostudies-literatureRecent applications of chemosensitivity tests for colorectal cancer treatment.PMC4248183Yoon YSKim JCfalseRecent applications of chemosensitivity tests for colorectal cancer treatment.The evaluation of therapeutic efficacy is necessary to predict the outcome of patients with metastatic colorectal cancer (CRC). In these patients, there is a critical need for predictive chemosensitivity assays and biomarkers to optimize efficacy and minimize toxicity. The introduction of targeted agents has improved the progression-free survival and overall survival of patients with metastatic disease. However, approximately 50% of patients do not show a positive response to chemotherapy and the selection of patients likely to respond to a specific regimen remains challenging. Cell culture-based chemosensitivity tests use autologous viable tumor cells to evaluate susceptibility to specific agents in vitro and predict their direct effects. Adenosine triphosphate-based assays and methyl thi2014-01-01T00:00:00Z2014 Nov2025-05-31T23:43:33.212Z2019-03-27T01:40:30ZS-EPMC42481832546900810.3748/wjg.v20.i44.16398