{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Smith SA"],"funding":["NIAID NIH HHS"],"pagination":["12233-41"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4248927"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(21)"],"pubmed_abstract":["<h4>Unlabelled</h4>Natural dengue virus (DENV) infection in humans induces antibodies (Abs) that neutralize the serotype of infection in a potent and type-specific manner; however, most Abs generated in response to infection are serotype cross-reactive and poorly neutralizing. Such cross-reactive Abs may enhance disease during subsequent infection with a virus of a different DENV serotype. Previous screening assays for DENV-specific human B cells and antibodies, using viral and recombinant antigens, mainly led to the isolation of dominant nonneutralizing B cell clones. To improve upon our ability to recover and study rare but durable and potently neutralizing DENV-specific Abs, we isolated human DENV-specific B cells by using a primary screen of binding to live virus, followed by a seconda"],"journal":["Journal of virology"],"pubmed_title":["Isolation of dengue virus-specific memory B cells with live virus antigen from human subjects following natural infection reveals the presence of diverse novel functional groups of antibody clones."],"pmcid":["PMC4248927"],"funding_grant_id":["R01 AI107731","U54 AI057157","K08 AI103038"],"pubmed_authors":["Jadi RS","Smith SA","Crowe JE","Kose N","de Silva AM","de Alwis AR"],"additional_accession":[]},"is_claimable":false,"name":"Isolation of dengue virus-specific memory B cells with live virus antigen from human subjects following natural infection reveals the presence of diverse novel functional groups of antibody clones.","description":"<h4>Unlabelled</h4>Natural dengue virus (DENV) infection in humans induces antibodies (Abs) that neutralize the serotype of infection in a potent and type-specific manner; however, most Abs generated in response to infection are serotype cross-reactive and poorly neutralizing. Such cross-reactive Abs may enhance disease during subsequent infection with a virus of a different DENV serotype. Previous screening assays for DENV-specific human B cells and antibodies, using viral and recombinant antigens, mainly led to the isolation of dominant nonneutralizing B cell clones. To improve upon our ability to recover and study rare but durable and potently neutralizing DENV-specific Abs, we isolated human DENV-specific B cells by using a primary screen of binding to live virus, followed by a seconda","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Nov","modification":"2026-05-07T02:45:24.993Z","creation":"2026-04-07T22:33:01.268Z"},"accession":"S-EPMC4248927","cross_references":{"pubmed":["25100837"],"doi":["10.1128/JVI.00247-14","10.1128/jvi.00247-14"]}}