biostudies-literatureSmith SANIAID NIH HHS12233-41https://www.ebi.ac.uk/biostudies/studies/S-EPMC4248927biostudies-literatureUnknown88(21)<h4>Unlabelled</h4>Natural dengue virus (DENV) infection in humans induces antibodies (Abs) that neutralize the serotype of infection in a potent and type-specific manner; however, most Abs generated in response to infection are serotype cross-reactive and poorly neutralizing. Such cross-reactive Abs may enhance disease during subsequent infection with a virus of a different DENV serotype. Previous screening assays for DENV-specific human B cells and antibodies, using viral and recombinant antigens, mainly led to the isolation of dominant nonneutralizing B cell clones. To improve upon our ability to recover and study rare but durable and potently neutralizing DENV-specific Abs, we isolated human DENV-specific B cells by using a primary screen of binding to live virus, followed by a secondaJournal of virologyIsolation of dengue virus-specific memory B cells with live virus antigen from human subjects following natural infection reveals the presence of diverse novel functional groups of antibody clones.PMC4248927R01 AI107731U54 AI057157K08 AI103038Jadi RSSmith SACrowe JEKose Nde Silva AMde Alwis ARfalseIsolation of dengue virus-specific memory B cells with live virus antigen from human subjects following natural infection reveals the presence of diverse novel functional groups of antibody clones.<h4>Unlabelled</h4>Natural dengue virus (DENV) infection in humans induces antibodies (Abs) that neutralize the serotype of infection in a potent and type-specific manner; however, most Abs generated in response to infection are serotype cross-reactive and poorly neutralizing. Such cross-reactive Abs may enhance disease during subsequent infection with a virus of a different DENV serotype. Previous screening assays for DENV-specific human B cells and antibodies, using viral and recombinant antigens, mainly led to the isolation of dominant nonneutralizing B cell clones. To improve upon our ability to recover and study rare but durable and potently neutralizing DENV-specific Abs, we isolated human DENV-specific B cells by using a primary screen of binding to live virus, followed by a seconda2014-01-01T00:00:00Z2014 Nov2026-05-07T02:45:24.993Z2026-04-07T22:33:01.268ZS-EPMC42489272510083710.1128/JVI.00247-1410.1128/jvi.00247-14