{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fu X"],"funding":["NCRR NIH HHS","NIGMS NIH HHS"],"pagination":["1875-1882"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4255137"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(12)"],"pubmed_abstract":["Cryo-electron tomography (cryoET) has become a powerful tool for direct visualization of 3D structures of native biological specimens at molecular resolution, but its application is limited to thin specimens (<300 nm). Recently, vitreous sectioning and cryoFIB milling technologies were developed to physically reduce the specimen thickness; however, cryoET analysis of membrane protein complexes within native cell membranes remains a great challenge. Here, we use phage ΦX174 lysis gene E to rapidly produce native, intact, bacterial cell membranes for high resolution cryoET. We characterized E gene-induced cell lysis using FIB/SEM and cryoEM and showed that the bacteria cytoplasm was largely depleted through spot lesion, producing ghosts with the cell membranes intact. We further demonstrated"],"journal":["Structure (London, England : 1993)"],"pubmed_title":["Controlled bacterial lysis for electron tomography of native cell membranes."],"pmcid":["PMC4255137"],"funding_grant_id":["C06 RR017528-01","GM085043","C06 RR017528","RR024424","R01 GM085043","R21 RR024424","S10 RR029300"],"pubmed_authors":["Himes BA","Fu X","Rice WJ","Ning J","Ke D","Zhang P"],"additional_accession":[]},"is_claimable":false,"name":"Controlled bacterial lysis for electron tomography of native cell membranes.","description":"Cryo-electron tomography (cryoET) has become a powerful tool for direct visualization of 3D structures of native biological specimens at molecular resolution, but its application is limited to thin specimens (<300 nm). Recently, vitreous sectioning and cryoFIB milling technologies were developed to physically reduce the specimen thickness; however, cryoET analysis of membrane protein complexes within native cell membranes remains a great challenge. Here, we use phage ΦX174 lysis gene E to rapidly produce native, intact, bacterial cell membranes for high resolution cryoET. We characterized E gene-induced cell lysis using FIB/SEM and cryoEM and showed that the bacteria cytoplasm was largely depleted through spot lesion, producing ghosts with the cell membranes intact. We further demonstrated","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Dec","modification":"2025-06-27T03:05:31.661Z","creation":"2025-06-27T03:05:31.661Z"},"accession":"S-EPMC4255137","cross_references":{"pubmed":["25456413"],"doi":["10.1016/j.str.2014.09.017"]}}