biostudies-literatureUnknown9(12)Chakraborty M<h4>Rationale</h4>The chemokine interleukin-8 is implicated in the development of bronchopulmonary dysplasia in preterm infants. The 77-amino acid isoform of interleukin-8 (interleukin-877) is a less potent chemoattractant than other shorter isoforms. Although interleukin-877 is abundant in the preterm circulation, its regulation in the preterm lung is unknown.<h4>Objectives</h4>To study expression and processing of pulmonary interleukin-877 in preterm infants who did and did not develop bronchopulmonary dysplasia.<h4>Methods</h4>Total interleukin-8 and interleukin-877 were measured in bronchoalveolar lavage fluid from preterm infants by immunoassay. Neutrophil serine proteases were used to assess processing. Neutrophil chemotaxis assays and degranulation of neutrophil matrix metalloproteiPloS onee114524https://www.ebi.ac.uk/biostudies/studies/S-EPMC4256433biostudies-literatureRole of serine proteases in the regulation of interleukin-877 during the development of bronchopulmonary dysplasia in preterm ventilated infants.PMC4256433Powell WDavies PLHogwood JGray EMcGreal EPSpiller BKotecha SVoitenok NNChambers RCWilliams AChakraborty MAbdulla SfalseRole of serine proteases in the regulation of interleukin-877 during the development of bronchopulmonary dysplasia in preterm ventilated infants.<h4>Rationale</h4>The chemokine interleukin-8 is implicated in the development of bronchopulmonary dysplasia in preterm infants. The 77-amino acid isoform of interleukin-8 (interleukin-877) is a less potent chemoattractant than other shorter isoforms. Although interleukin-877 is abundant in the preterm circulation, its regulation in the preterm lung is unknown.<h4>Objectives</h4>To study expression and processing of pulmonary interleukin-877 in preterm infants who did and did not develop bronchopulmonary dysplasia.<h4>Methods</h4>Total interleukin-8 and interleukin-877 were measured in bronchoalveolar lavage fluid from preterm infants by immunoassay. Neutrophil serine proteases were used to assess processing. Neutrophil chemotaxis assays and degranulation of neutrophil matrix metalloprotei2014-01-01T00:00:00Z20142026-07-16T07:27:20.399Z2019-03-26T23:25:08ZS-EPMC42564332547441210.1371/journal.pone.0114524