biostudies-literatureChen PBLRD VANIDDK NIH HHSNIAAA NIH HHS203-214.e16https://www.ebi.ac.uk/biostudies/studies/S-EPMC4274236biostudies-literatureUnknown148(1)<h4>Background & aims</h4>Alcoholic liver disease is a leading cause of mortality. Chronic alcohol consumption is accompanied by intestinal dysbiosis, and development of alcoholic liver disease requires gut-derived bacterial products. However, little is known about how alterations to the microbiome contribute to pathogenesis of alcoholic liver disease.<h4>Methods</h4>We used the Tsukamoto-French mouse model, which involves continuous intragastric feeding of isocaloric diet or alcohol for 3 weeks. Bacterial DNA from the cecum was extracted for deep metagenomic sequencing. Targeted metabolomics assessed concentrations of saturated fatty acids in cecal contents. To maintain intestinal metabolic homeostasis, diets of ethanol-fed and control mice were supplemented with saturated long-chain fattGastroenterologySupplementation of saturated long-chain fatty acids maintains intestinal eubiosis and reduces ethanol-induced liver injury in mice.PMC4274236R01 AA020216K23 DK090303R01 AA020203I01 BX002213K08 DK081830U24 DK097153P50 AA011999RC2 AA019405U01 AA021856R01 AA020703Starkel PNelson KEvan Pijkeren JPHo SBChen PTorralba MTan JBajaj JSMutlu EAEmbree MTsukamoto HKeshavarzian ASchnabl BFouts DEDePew JLoomba RZengler KfalseSupplementation of saturated long-chain fatty acids maintains intestinal eubiosis and reduces ethanol-induced liver injury in mice.<h4>Background & aims</h4>Alcoholic liver disease is a leading cause of mortality. Chronic alcohol consumption is accompanied by intestinal dysbiosis, and development of alcoholic liver disease requires gut-derived bacterial products. However, little is known about how alterations to the microbiome contribute to pathogenesis of alcoholic liver disease.<h4>Methods</h4>We used the Tsukamoto-French mouse model, which involves continuous intragastric feeding of isocaloric diet or alcohol for 3 weeks. Bacterial DNA from the cecum was extracted for deep metagenomic sequencing. Targeted metabolomics assessed concentrations of saturated fatty acids in cecal contents. To maintain intestinal metabolic homeostasis, diets of ethanol-fed and control mice were supplemented with saturated long-chain fatt2015-01-01T00:00:00Z2015 Jan2026-06-04T00:39:33.502Z2019-03-27T01:42:13ZS-EPMC42742362523959110.1053/j.gastro.2014.09.014