{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["70(6)"],"submitter":["Fey A"],"pubmed_abstract":["Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping genes does not occur, and the use of DNA standards leads to a broad underestimation. Therefore, an accurate quantification of RNA is feasible only by using appropriate RNA standards. We established and validated a Q-PCR method which enables the quantification of not only the number of copies of target genes (i.e., the number of bacterial cells) but also the number of RNA copies. The genes coding for InvA and the 16S rRNA of Salmonella enterica serovar Typhimurium were selected for the evaluation"],"journal":["Applied and environmental microbiology"],"pagination":["3618-23"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC427797"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism."],"pmcid":["PMC427797"],"pubmed_authors":["Fey A","Christen R","Hofle MG","Guzman CA","Flavier S","Eichler S"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism.","description":"Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping genes does not occur, and the use of DNA standards leads to a broad underestimation. Therefore, an accurate quantification of RNA is feasible only by using appropriate RNA standards. We established and validated a Q-PCR method which enables the quantification of not only the number of copies of target genes (i.e., the number of bacterial cells) but also the number of RNA copies. The genes coding for InvA and the 16S rRNA of Salmonella enterica serovar Typhimurium were selected for the evaluation","dates":{"release":"2004-01-01T00:00:00Z","publication":"2004 Jun","modification":"2025-05-29T20:32:10.3Z","creation":"2019-03-27T00:50:32Z"},"accession":"S-EPMC427797","cross_references":{"pubmed":["15184165"],"doi":["10.1128/AEM.70.6.3618-3623.2004","10.1128/aem.70.6.3618-3623.2004"]}}