{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["1(10)"],"submitter":["Melo-Lima S"],"pubmed_abstract":["Necroptosis is a regulated necrotic cell death that involves receptor-interacting protein kinases RIPK1 and RIPK3. Here, we report that edelfosine triggers a rapid and massive cell death in human glioblastoma cells with characteristics of necrosis. Only a minor proportion of edelfosine-treated cells underwent caspase-dependent apoptosis. Autophagy and a rapid influx of extracellular calcium into the cells had little impact on cell death. Levels of procaspase-8 were very low in necroptosis-prone glioma cells compared with the levels in other cancer cell types that underwent apoptosis upon edelfosine treatment. The RIPK1-dependent necroptosis inhibitors necrostatin-1 (Nec-1) and Nec-1s as well as siRNA-mediated silencing of RIPK3 inhibited edelfosine-induced necroptosis, resulting in increas"],"journal":["Oncoscience"],"pagination":["649-64"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4278276"],"repository":["biostudies-literature"],"pubmed_title":["Necroptosis is associated with low procaspase-8 and active RIPK1 and -3 in human glioma cells."],"pmcid":["PMC4278276"],"pubmed_authors":["Celeste Lopes M","Melo-Lima S","Mollinedo F"],"additional_accession":[]},"is_claimable":false,"name":"Necroptosis is associated with low procaspase-8 and active RIPK1 and -3 in human glioma cells.","description":"Necroptosis is a regulated necrotic cell death that involves receptor-interacting protein kinases RIPK1 and RIPK3. Here, we report that edelfosine triggers a rapid and massive cell death in human glioblastoma cells with characteristics of necrosis. Only a minor proportion of edelfosine-treated cells underwent caspase-dependent apoptosis. Autophagy and a rapid influx of extracellular calcium into the cells had little impact on cell death. Levels of procaspase-8 were very low in necroptosis-prone glioma cells compared with the levels in other cancer cell types that underwent apoptosis upon edelfosine treatment. The RIPK1-dependent necroptosis inhibitors necrostatin-1 (Nec-1) and Nec-1s as well as siRNA-mediated silencing of RIPK3 inhibited edelfosine-induced necroptosis, resulting in increas","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-20T02:51:11.398Z","creation":"2019-03-27T01:42:27Z"},"accession":"S-EPMC4278276","cross_references":{"pubmed":["25593994"],"doi":["10.18632/oncoscience.89"]}}