biostudies-literatureDunn WBMedical Research CouncilBiotechnology and Biological Sciences Research Council9-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC4289517biostudies-literatureUnknown11Phenotyping of 1,200 'healthy' adults from the UK has been performed through the investigation of diverse classes of hydrophilic and lipophilic metabolites present in serum by applying a series of chromatography-mass spectrometry platforms. These data were made robust to instrumental drift by numerical correction; this was prerequisite to allow detection of subtle metabolic differences. The variation in observed metabolite relative concentrations between the 1,200 subjects ranged from less than 5 % to more than 200 %. Variations in metabolites could be related to differences in gender, age, BMI, blood pressure, and smoking. Investigations suggest that a sample size of 600 subjects is both necessary and sufficient for robust analysis of these data. Overall, this is a large scale and non-tarMetabolomics : Official journal of the Metabolomic SocietyMolecular phenotyping of a UK population: defining the human serum metabolome.PMC4289517MC_QA137293BB/C51902X/1BB/C519038/1McWhirter CLin WKnowles JDPotter PPurandare NTseng AVaughan AAEllis DIZelena EHarding NJayson GCKell DBDunn WBThornton PZubair MO'Hagan SHaselden JNHalsall AWu FCPotts CBroadhurst DFrancis-McIntyre SChew-Graham SWinder CLAarons GWilson IDBegley PBenjamin BGoodacre RMoseley CBrown MFinn JDNicholls AWBurns ACruickshank JKPan MfalseMolecular phenotyping of a UK population: defining the human serum metabolome.Phenotyping of 1,200 'healthy' adults from the UK has been performed through the investigation of diverse classes of hydrophilic and lipophilic metabolites present in serum by applying a series of chromatography-mass spectrometry platforms. These data were made robust to instrumental drift by numerical correction; this was prerequisite to allow detection of subtle metabolic differences. The variation in observed metabolite relative concentrations between the 1,200 subjects ranged from less than 5 % to more than 200 %. Variations in metabolites could be related to differences in gender, age, BMI, blood pressure, and smoking. Investigations suggest that a sample size of 600 subjects is both necessary and sufficient for robust analysis of these data. Overall, this is a large scale and non-tar2015-01-01T00:00:00Z20152026-07-16T10:47:58.743Z2019-03-27T01:43:12ZS-EPMC42895172559876410.1007/s11306-014-0707-1