{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["171(21)"],"submitter":["Omori K"],"funding":["Mochida Memorial Foundation","Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)","JSPS Fellows","Takeda Science Foundation","Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry","Japan Society for the Promotion of Science","Towa Foundation"],"pubmed_abstract":["<h4>Background and purpose</h4>PGE2 is a major prostanoid that regulates inflammation by stimulating EP1-4 receptors. However, how PGE2 induces an initial inflammatory response to vascular hyper-permeability remains unknown. Here we investigated the role of the PGE2 -EP receptor signal in modulating vascular permeability both in vivo and in vitro.<h4>Experimental approach</h4>We used a modified Miles assay and intravital microscopy to examine vascular permeability in vivo. Endothelial barrier property was assessed by measuring transendothelial electrical resistance (TER) in vitro.<h4>Key results</h4>Local administration of PGE2 , an EP2 or EP4 receptor agonist into FVB/NJcl mouse ear skin caused vascular leakage, indicated by dye extravasation. Intravital microscopy and laser Doppler blood"],"journal":["British journal of pharmacology"],"pagination":["4879-89"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4294111"],"repository":["biostudies-literature"],"pubmed_title":["Multiple roles of the PGE2 -EP receptor signal in vascular permeability."],"pmcid":["PMC4294111"],"pubmed_authors":["Murata T","Hori M","Ozaki H","Omori K","Kida T"],"additional_accession":[]},"is_claimable":false,"name":"Multiple roles of the PGE2 -EP receptor signal in vascular permeability.","description":"<h4>Background and purpose</h4>PGE2 is a major prostanoid that regulates inflammation by stimulating EP1-4 receptors. However, how PGE2 induces an initial inflammatory response to vascular hyper-permeability remains unknown. Here we investigated the role of the PGE2 -EP receptor signal in modulating vascular permeability both in vivo and in vitro.<h4>Experimental approach</h4>We used a modified Miles assay and intravital microscopy to examine vascular permeability in vivo. Endothelial barrier property was assessed by measuring transendothelial electrical resistance (TER) in vitro.<h4>Key results</h4>Local administration of PGE2 , an EP2 or EP4 receptor agonist into FVB/NJcl mouse ear skin caused vascular leakage, indicated by dye extravasation. Intravital microscopy and laser Doppler blood","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Nov","modification":"2025-05-29T21:22:07.623Z","creation":"2025-05-29T21:22:07.623Z"},"accession":"S-EPMC4294111","cross_references":{"pubmed":["24923772"],"doi":["10.1111/bph.12815"]}}